Application of a Vacuum Pressure Impregnation Technique for Rehydrating Decellularized Tissues
Application of a Vacuum Pressure Impregnation Technique for Rehydrating Decellularized Tissues
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DOI：
10.1089/ten.tec.2013.0654
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发表时间：
2014-09-01
影响因子：
3
通讯作者：
Kishida, Akio
中科院分区：
文献类型：
--
作者：
Negishi, Jun;Hashimoto, Yoshihide;Kishida, Akio
大多数临床上可用的脱细胞组织以冻干状态保存。冷冻干燥（FD）组织可以长期保存，尽管在使用前需要进行再水化过程。目前，浸泡法是临床上最常用的方法，但对于复杂和厚结构的组织再水化是困难的。在这项研究中，我们试图应用真空压力浸渍（VPI）技术FD组织再水化。使用VPI，脱细胞组织的含水量可以在30 min内达到天然组织的含水量，而使用浸泡方法需要6 h才能达到相同的含水量。此外，肝素再水化的血管VPI有更多的肝素释放在每个时间点，因此出现更大的抗凝活性。我们发现，VPI处理促进溶液渗透到材料中，实现脱细胞组织的完全再水化，以及肝素深度渗透到脱细胞组织中，这表明VPI处理可用作生物材料的再水化方法。
Most of the clinically available decellularized tissues are preserved in a freeze-dried state. Freeze-dried (FD) tissues can be preserved for long term, although a rehydration process is necessary before use. Currently, an immersion method is most commonly used in clinical procedures, but it is difficult for complicated and thick structure tissue rehydration. In this study, we tried to apply a vacuum pressure impregnation (VPI) technique for FD tissue rehydration. The water content of decellularized tissues can reach the water content of native tissues within 30 min using VPI, whereas it took 6 h to reach the same water content using the immersion method. Furthermore, heparin rehydrated aortas by VPI had more heparin release at each time point and therefore appeared more anticoagulant activity. We found that the VPI treatment promotes solution infiltration into materials, achieves complete rehydration of the decellularized tissues, and deep infiltration of heparin into the decellularized tissues, suggesting that VPI treatment could be applied as a rehydration method for biological materials.