The genome of the emerging barley pathogen Ramularia collo-cygni.
The genome of the emerging barley pathogen Ramularia collo-cygni.
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DOI：
10.1186/s12864-016-2928-3
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发表时间：
2016-08-09
期刊：
影响因子：
4.4
通讯作者：
Havis ND
中科院分区：
文献类型：
--
作者：
McGrann GR;Andongabo A;Sjökvist E;Trivedi U;Dussart F;Kaczmarek M;Mackenzie A;Fountaine JM;Taylor JM;Paterson LJ;Gorniak K;Burnett F;Kanyuka K;Hammond-Kosack KE;Rudd JJ;Blaxter M;Havis ND
青枯菌是引起大麦叶斑病的一种新的重要叶面真菌。在改变生活习性成为侵袭性、坏死性病原菌之前，该真菌表现出延长的内生生长阶段，从而对绿叶面积造成重大损失，从而导致粮食产量和品质的损失。使用Illumina和罗氏454技术的组合对霍乱弧菌的基因组进行了测序。30.3 Mb的草案组装包含11,617个预测基因模型。我们的系统发育分析证实了这种子囊菌的分类属于丝孢菌纲的顶孢菌目Mycsphaerellaceae。预测的分泌组由1053个蛋白质组成，包括氧化还原相关酶、碳水化合物修饰酶和蛋白酶。植物细胞壁降解酶基因的相对缺乏可能与真菌科植物病原菌的隐形致病特性有关。鉴定了大量与次生代谢产物产生相关的基因，包括在其他Dothidemycae植物病原菌中发现的毒素生物合成基因的同源物。Colo-Cygni的基因组序列为理解这种重要的新兴病原体的发病机制提供了一个框架。碳水化合物降解酶基因的减少可能反映了一种策略，以避免在其长期无症状生长期间被宿主防御检测到。特别令人感兴趣的是分析R.colo-Cygni在与宿主大麦相互作用过程中的基因表达，以了解是什么触发这种真菌从良性内生菌转变为侵略性的坏死菌。本文的在线版本(doi：10.1186/s12864-0162928-3)包含补充材料，授权用户可以使用。
Ramularia collo-cygni is a newly important, foliar fungal pathogen of barley that causes the disease Ramularia leaf spot. The fungus exhibits a prolonged endophytic growth stage before switching life habit to become an aggressive, necrotrophic pathogen that causes significant losses to green leaf area and hence grain yield and quality. The R. collo-cygni genome was sequenced using a combination of Illumina and Roche 454 technologies. The draft assembly of 30.3 Mb contained 11,617 predicted gene models. Our phylogenomic analysis confirmed the classification of this ascomycete fungus within the family Mycosphaerellaceae, order Capnodiales of the class Dothideomycetes. A predicted secretome comprising 1053 proteins included redox-related enzymes and carbohydrate-modifying enzymes and proteases. The relative paucity of plant cell wall degrading enzyme genes may be associated with the stealth pathogenesis characteristic of plant pathogens from the Mycosphaerellaceae. A large number of genes associated with secondary metabolite production, including homologs of toxin biosynthesis genes found in other Dothideomycete plant pathogens, were identified. The genome sequence of R. collo-cygni provides a framework for understanding the genetic basis of pathogenesis in this important emerging pathogen. The reduced complement of carbohydrate-degrading enzyme genes is likely to reflect a strategy to avoid detection by host defences during its prolonged asymptomatic growth. Of particular interest will be the analysis of R. collo-cygni gene expression during interactions with the host barley, to understand what triggers this fungus to switch from being a benign endophyte to an aggressive necrotroph. The online version of this article (doi:10.1186/s12864-016-2928-3) contains supplementary material, which is available to authorized users.