Tanshinone production and isoprenoid pathways in Salvia miltiorrhiza hairy roots induced by Ag+ and yeast elicitor
Tanshinone production and isoprenoid pathways in Salvia miltiorrhiza hairy roots induced by Ag+ and yeast elicitor
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DOI：
10.1016/j.plantsci.2004.09.012
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发表时间：
2005-02-01
期刊：
影响因子：
5.2
通讯作者：
Wu, JY
中科院分区：
文献类型：
--
作者：
Ge, XC;Wu, JY
本研究研究了生物激发剂酵母提取物的碳水化合物部分（YE， 100马克毫升（-1））和非生物激发剂Ag+（30毫升）诱导丹参酮二萜在丹参酮毛状根培养中的积累和相关的次级代谢途径。Ag+只刺激3-羟基-3-甲基戊二酰辅酶a还原酶（HMGR）活性，而Ag+和YE均刺激1-脱氧-木糖5-磷酸合酶（DXS）活性，但YE的刺激作用更强。非mva途径抑制剂fosmidomycin抑制两种激发子诱导的丹参酮积累，而mva途径抑制剂mevinolin仅抑制a诱导的丹参酮积累。结果表明，两种激发子诱导的丹参酮积累主要通过非MVA途径（DXS活性）合成，但可能依赖于MVA和非MVA途径之间的串扰。此外，用Ag+预处理毛状根24-48 h可增强叶黄诱导的丹参酮产量和DXS活性。2004爱思唯尔爱尔兰有限公司版权所有。
This work examined the accumulation of diterpenoid tanshinones and related secondary metabolism pathways in hairy root cultures of Salvia miltiorrhiza Bunge (Lamiacae) induced by a biotic elicitor, the carbohydrate fraction of yeast extract (YE, 100 mug mL(-1)), and an abiotic elicitor, Ag+ (30 muM). The activity of 3-hydroxy-3-methylglutaryl CoA redutase (HMGR) was only stimulated by Ag+, and 1-deoxyD-xylulose 5-phosphate synthase (DXS) was stimulated by both, but more strongly by YE. While the non-MVA pathway inhibitor fosmidomycin inhibited the tanshinone accumulation induced by both elicitors, the MVA-pathway inhibitor mevinolin only suppressed the A-induced tanshinone accumulation. The results suggest that the tanshinone accumulation induced by the two elicitors was mainly synthesized via the non-MVA pathway (DXS activity), but could depend on crosstalk between the MVA and non-MVA pathways. Furthermore, the pretreatment of hairy roots with Ag+ for 24-48 h potentiated the YE-induced tanshinone production and DXS activity. (C) 2004 Elsevier Ireland Ltd. All rights reserved.