Identification of the sequence required for expression of the 2H4 epitope on the human leukocyte common antigens.
Identification of the sequence required for expression of the 2H4 epitope on the human leukocyte common antigens.
复制标题
鉴定人类白细胞共同抗原上 2H4 表位表达所需的序列。
DOI：
10.1084/jem.166.5.1567
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Saito,H
中科院分区：
文献类型：
--
作者：
Streuli,M;Matsuyama,T;Morimoto,C;Schlossman,SF;Saito,H
抗2 H4抗体将人T淋巴细胞的T4+群体细分为T4+，2 H4+抑制-诱导细胞和T4+，2 H4-辅助细胞，识别人白细胞共同抗原（LCA）亚群上的表位。LCA是由单个基因通过NH 2末端附近的三个外显子的差异使用产生的细胞表面糖蛋白家族。使用对应于四种不同形式的LCA分子的cDNA克隆，在体外合成LCA分子的胞外结构域。这些分子与抗2 H4抗体的免疫沉淀证明了外显子A是2 H4表位表达所需的。
The anti-2H4 antibody, which subdivides the T4+ population of human T lymphocytes into T4+, 2H4+ suppressor-inducer cells and T4+, 2H4- helper cells, recognizes an epitope on a subset of the human leukocyte common antigens (LCAs). LCAs are a family of cell surface glycoproteins generated from a single gene by the differential usage of three exons near the NH2-terminus. Using cDNA clones corresponding to four of the different forms of LCA molecules, extracellular domains of the LCA molecules were synthesized in vitro. Immunoprecipitation of these molecules with the anti-2H4 antibody demonstrated that exon A is required for the expression of the 2H4 epitope.