The arbuscular mycorrhizal symbiosis promotes the systemic induction of regulatory defence-related genes in rice leaves and confers resistance to pathogen infection
The arbuscular mycorrhizal symbiosis promotes the systemic induction of regulatory defence-related genes in rice leaves and confers resistance to pathogen infection
复制标题
DOI：
10.1111/j.1364-3703.2011.00773.x
复制
发表时间：
2012-08-01
影响因子：
4.9
通讯作者：
San Segundo, Blanca
中科院分区：
文献类型：
--
作者：
Campos-Soriano, Lidia;Garcia-Martinez, Jose;San Segundo, Blanca
丛枝菌根(AM)共生是土壤真菌与大多数维管植物之间的互惠共生关系。它们的联合通过改善营养，主要是磷营养，并提供更强的应对不利条件的能力，使寄主植物受益。在这项研究中，我们研究了AM共生在水稻叶片中引发的转录变化，重点是植物防御反应的相关性。我们发现AM真菌的根部定殖伴随着在寄主防御反应中起调节作用的基因的系统诱导，如OsNPR1、OsAP2、OsEREBP和OsJAmyb。参与信号转导过程的基因(OsDUF26和OsMPK6)和参与钙介导的信号转导过程的基因(OsCBP、OsCaM和OsCML4)也在没有病原菌侵染的情况下在菌根水稻的叶片中上调。此外，菌根水稻植株在叶片中表现出更强的防御标记基因[即病程相关(PR)基因]，以响应稻瘟病菌的侵染。有证据表明，菌根水稻植株对稻瘟病菌表现出较强的抗性。总之，这些结果表明，AM共生在水稻植株中的保护作用既依赖于在没有病原菌攻击的情况下防御调节基因的系统激活，也依赖于在病原菌侵染过程中防御效应基因的更强表达。讨论了菌根诱导对稻瘟病菌侵染的抗性机制。
Arbuscular mycorrhizal (AM) symbioses are mutualistic associations between soil fungi and most vascular plants. Their association benefits the host plant by improving nutrition, mainly phosphorus nutrition, and by providing increased capability to cope with adverse conditions. In this study, we investigated the transcriptional changes triggered in rice leaves as a result of AM symbiosis, focusing on the relevance of the plant defence response. We showed that root colonization by the AM fungus Glomus intraradices is accompanied by the systemic induction of genes that play a regulatory role in the host defence response, such as OsNPR1, OsAP2, OsEREBP and OsJAmyb. Genes involved in signal transduction processes (OsDUF26 and OsMPK6) and genes that function in calcium-mediated signalling processes (OsCBP, OsCaM and OsCML4) are also up-regulated in leaves of mycorrhizal rice plants in the absence of pathogen infection. In addition, the mycorrhizal rice plants exhibit a stronger induction of defence marker genes [i.e. pathogenesis-related (PR) genes] in their leaves in response to infection by the blast fungus Magnaporthe oryzae. Evidence indicates that mycorrhizal rice plants show enhanced resistance to the rice blast fungus. Overall, these results suggest that the protective effect of the AM symbiosis in rice plants relies on both the systemic activation of defence regulatory genes in the absence of pathogen challenge and the priming for stronger expression of defence effector genes during pathogen infection. The possible mechanisms involved in the mycorrhiza-induced resistance to M. oryzae infection are discussed.