Rab11 regulates E-cadherin expression and induces cell transformation in colorectal carcinoma.
Rab11 regulates E-cadherin expression and induces cell transformation in colorectal carcinoma.
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RAB11调节e-钙粘蛋白的表达并诱导大肠癌中的细胞转化。
DOI：
10.1186/1471-2407-14-587
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发表时间：
2014-08-12
期刊：
影响因子：
3.8
通讯作者：
Chao WT
中科院分区：
文献类型：
--
作者：
Chung YC;Wei WC;Huang SH;Shih CM;Hsu CP;Chang KJ;Chao WT
在上皮细胞间充质转化过程中，E-钙粘附素等连接黏附复合体的解体是细胞形态和极性改变的显著标志。然而，E-钙粘蛋白的表达是动态的，受细胞内吞系统的调节，也参与细胞信号转导机制。在本研究中，我们研究了E-钙粘蛋白在大肠肿瘤中的作用以及在结肠细胞转化过程中与循环内体蛋白Rab11的关系。应用免疫组织化学方法检测113例结直肠癌组织中E-钙粘蛋白和Rab11的表达。在体外细胞实验中，将GFP标记的Rab11载体导入HT29结肠癌细胞，用Western印迹和共聚焦显微镜观察E-钙粘蛋白的表达和细胞转化情况。免疫组织化学染色显示，E-钙粘蛋白在肿瘤组织和正常组织中的平均积分分别为1.41 ± 0.06和1.08 ± 0.06(p <； 0.05)。肿瘤组织和正常组织中Rab11的平均评分分别为0.51 ± 0.05和0.18 ± 0.02(p <； 0.05)。74例(66.5%)结直肠癌组织中E-钙粘蛋白和Rab11同步过表达。GFP标记的Rab11在体外培养的结肠癌细胞株HT-29中过表达时，E-钙粘附素表达上调，并诱导细胞膜突起，导致细胞转化和迁移。本研究证实了Rab11和E-钙粘蛋白在结直肠癌中过表达的重要性。结果表明，Rab11和E-钙粘蛋白可能是结直肠癌进展和治疗的潜在标记物。
In the process of epithelial mesenchymal transition EMT, the disassembly of junctional adhesion complexes such as E-cadherin is a remarkable sign during changes in cell morphology and polarity. However, E-cadherin expression is dynamic, and is regulated by the cellular endocytic system; it is also involved in cell signaling mechanisms. In this study, we investigated the role of E-cadherin in colorectal tumors and the relationship with recycling endosome protein Rab11 in colon cell transformation. For tissue screening, the expressions of E-cadherin and Rab11 in colorectal tumors were identified by immunohistochemistry in 113 patients with colorectal carcinoma. For the in vitro cell experiment, GFP-tagged Rab11 plasmid was transfected into HT29 colon cells, E-cadherin expression and cell transformation were monitored by Western blot and confocal microscopy. In immunohistochemistry, the mean score of E-cadherin in tumor and normal tissues was 1.41 ± 0.06 and 1.08 ± 0.06 (p < 0.05). The mean score of Rab11 in tumor and normal tissues was 0.51 ± 0.05 and 0.18 ± 0.02 (p < 0.05). Synchronous overexpression of E-cadherin and Rab11 was noted in 74 patients (66.5%) with colorectal carcinoma. When GFP-tagged Rab11 plasmid was overexpressed in cultured colon cell line HT-29, the E-cadherin expression was up-regulated, and cell membrane protrusion was induced, which resulted in cell transformation and cell migration. This study demonstrated the importance of the overexpression of Rab11 and E-cadherin in colorectal cancer. The results indicated that Rab11 together with E-cadherin might be potential markers for colorectal cancer progression and treatment.
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影响因子：
3.3
作者：
Ducharme, Nicole A.;Hales, Chadwick M.;Goldenring, James R.
通讯作者：
Goldenring, James R.
电子 - 钙黏着蛋白的阴暗面：可能在肿瘤进展中的作用。
影响因子：
11.2
作者：
Rodriguez, Fausto J.;Lewis-Tuffin, Laura J.;Anastasiadis, Panos Z.
通讯作者：
Anastasiadis, Panos Z.
影响因子：
3.7
作者：
Kachhap SK;Faith D;Qian DZ;Shabbeer S;Galloway NL;Pili R;Denmeade SR;DeMarzo AM;Carducci MA
通讯作者：
Carducci MA
影响因子：
37.3
作者：
Chao YL;Shepard CR;Wells A
通讯作者：
Wells A
DOI：
10.1083/jcb.200705094
发表时间：
2007-07-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nejsum LN;Nelson WJ
通讯作者：
Nelson WJ