FRACTIONATION OF SIALYL OLIGOSACCHARIDES OF HUMAN MILK BY ION-EXCHANGE CHROMATOGRAPHY
FRACTIONATION OF SIALYL OLIGOSACCHARIDES OF HUMAN MILK BY ION-EXCHANGE CHROMATOGRAPHY
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DOI：
10.1016/0003-2697(78)90261-0
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发表时间：
1978-01-01
影响因子：
2.9
通讯作者：
GINSBURG, V
中科院分区：
文献类型：
--
作者：
SMITH, DF;ZOPF, DA;GINSBURG, V
通过在醋酸-吡啶缓冲液（pH 5.4）中的 DEAE-纤维素上进行离子交换色谱法，部分分离母乳中的唾液酸寡糖。以基本上定量的产率获得了一种中性和7种唾液酸寡糖级分。该方法允许大规模制备唾液酸寡糖级分，从中可以纯化单独的唾液酸寡糖，而无需制备型高压纸电泳。
The sialyl oligosaccharides of human milk are partially separated by ion-exchange chromatography on DEAE-cellulose in acetic acid-pyridine buffers, pH 5.4. One neutral and 7 sialyl oligosaccharide fractions are obtained in essentially quantitative yields. The method permits the large-scale preparation of sialyl oligosaccharide fractions from which individual sialyl oligosaccharides may be purified without the necessity of preparative high-voltage paper electrophoresis.