Structure of the linkage-region between polysaccharide chain and core protein in bovine corneal proteokeratan sulfate.
Structure of the linkage-region between polysaccharide chain and core protein in bovine corneal proteokeratan sulfate.
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牛角膜硫酸蛋白角蛋白多糖链与核心蛋白之间连接区的结构。
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发表时间：
1982
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通讯作者：
H. Scharf
中科院分区：
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作者：
T. Stein;R. Keller;H. Stuhlsatz;H. Greiling;E. Ohst;E. Müller;H. Scharf
以牛角膜为原料，经水解、酶外消化和内切-β-N-乙酰氨基葡萄糖苷酶D消化，得到硫酸肽角蛋白。用[3 H]岩藻糖标记和[3 H]甘露糖标记的硫酸肽角蛋白进行相同的程序。通过在不同降解步骤对肽角蛋白进行甲基化分析以及内切-β-N-乙酰氨基葡糖苷酶D的作用获得的数据表明，来自牛角膜的硫酸蛋白角蛋白中的结合区域与各种GlcNAc（β 1-N）-Asn连接的甘露糖基糖蛋白中发现的结构相同。通过内切-β-N-乙酰氨基葡萄糖苷酶D的作用证明了天冬酰胺和甘露糖之间存在一个壳二糖单元。通过α-岩藻糖苷酶、内切-β-N-乙酰氨基葡萄糖苷酶D的作用和Bio-Gel P-4上的凝胶色谱法，证明了Asn结合GlcNAc处（α 1导致6）-连接岩藻糖基残基的存在和位置。通过气相色谱/质谱联用研究表明，结合区寡糖中除了1，4-二取代的GlcNAc外，还存在1，4，6-三取代的GlcNAc。本文报道的其他结果是根据先前公布的分析数据（Keller，R.，Stein，T.，Stuhlleland，H.W.，Greiling，H.，Ohst，E.，Müller，E. & Scharf，H.- D. 03 The Dog of the Dog（1981）Physiol.Chem.362，327-336）。
Peptidokeratan sulfate from bovine cornea was degraded by a combination of desulfation, exo-enzymic digestion and finally digestion with endo-beta-N-acetylglucosaminidase D. The same procedure was carried out both with [3H]fucose-labelled and [3H]mannose-labelled peptidokeratan sulfate. Data obtained by methylation analysis of peptidokeratan at the different degradation steps, as well as action of endo-beta-N-acetylglucosaminidase D, showed that the binding-region in proteokeratan sulfate from bovine cornea is identical with a structure found in various GlcNAc(beta 1-N)-Asn-linked mannosyl glycoproteins. The existence of a chitobiose unit between asparagine and mannose was proved by action of endo-beta-N-acetylglucosaminidase D. The existence and position of an (alpha 1 leads to 6)-linked fucosyl residue at the Asn-bound GlcNAc was demonstrated by action of alpha-fucosidase, endo-beta-N-acetylglucosaminidase D and by gel chromatography on Bio-Gel P-4. By gas chromatography/mass spectrometry studies, the existence of a 1,4,6-trisubstituted beside a 1,4-disubstituted GlcNAc in the binding-region oligosaccharide was shown. Other results reported here are according to analytical data previously published (Keller, R., Stein, T., Stuhlsatz, H.W., Greiling, H., Ohst, E., Müller, E. & Scharf, H.-D. (1981) Hoppe-Seyler's Z. Physiol. Chem. 362, 327-336).