Polymeric vector-mediated gene transfection of MSCs for dual bioluminescent and MRI tracking in vivo
Polymeric vector-mediated gene transfection of MSCs for dual bioluminescent and MRI tracking in vivo
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聚合载体介导的 MSC 基因转染，用于体内双生物发光和 MRI 追踪
DOI：
10.1016/j.biomaterials.2014.06.014
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发表时间：
2014-09-01
期刊：
影响因子：
14
通讯作者：
Shan, Hong
中科院分区：
文献类型：
--
作者：
Wu, Chun;Li, Jingguo;Shan, Hong
骨髓间充质干细胞移植是再生医学中一种很有前途的基于细胞的损伤治疗方法，利用无创技术观察移植骨髓间充质干细胞的体内可视化是跟踪细胞输注和归巢的必要条件。构建了一种新型阳离子聚合物聚乙二醇-聚l -天冬氨酸嵌段接枝聚乙烯亚胺超顺磁性氧化铁纳米颗粒（PAI/SPION），作为磁共振成像（MRI）可见的非病毒载体，用于将编码荧光素酶和红色荧光蛋白（RFP）的质粒DNA （pDNA）作为报告基因传递到间质干细胞中。结果，MSCs被SPION和报告基因标记。PAI/SPION复合物在将pDNA转移到MSCs中表现出较高的转染效率，从而导致荧光素酶和RFP的高效共表达。此外，这些复合物对MSCs的生存能力和多系分化能力没有显著影响。将标记的间充质干细胞经肠系膜上静脉（SMV）注射移植到急性肝损伤大鼠体内后，利用活体成像系统（IVIS）和磁共振成像（MRI）可以有效监测细胞的迁移行为和器官特异性积累。免疫组化分析进一步证实移植的间充质干细胞主要分布于肝实质。结果表明，PAI/SPION是一种MRI可见基因递送剂，可以有效地标记MSCs，为体内双峰生物发光和MRI跟踪提供基础。(C) 2014 Elsevier Ltd.版权所有。
MSC's transplantation is a promising cell-based therapy for injuries in regenerative medicine, and in vivo visualization of transplanted MSCs with noninvasive technique is essential for the tracking of cell infusion and homing. A new cationic polymer, poly(ethylene glycol)-block-poly(L-aspartic acid)-grafted polyethylenimine functionalized with superparamagnetic iron oxide nanoparticles (PAI/SPION), was constructed as a magnetic resonance imaging (MRI)-visible non-viral vector for the delivery of plasmids DNA (pDNA) encoding for luciferase and red fluorescence protein (RFP) as reporter genes into MSCs. As a result, the MSCs were labeled with SPION and reporter genes. The PAI/SPION complexes exhibited high transfection efficiency in transferring pDNA into MSCs, which resulted in efficient luciferase and RFP co-expression. Furthermore, the complexes did not significantly affect the viability and multilineage differentiation capacity of MSCs. After the labeled MSCs were transplanted into the rats with acute liver injury via the superior mesenteric vein (SMV) injection, the migration behavior and organ-specific accumulation of the cells could be effectively monitored using the in vivo imaging system (IVIS) and MRI, respectively. The immunohistochemical analysis further confirmed that the transplanted MSCs were predominantly distributed in the liver parenchyma. Our results indicate that the PAI/SPION is a MRI-visible gene delivery agent which can effectively label MSCs to provide the basis for bimodal bioluminescence and MRI tracking in vivo. (C) 2014 Elsevier Ltd. All rights reserved.