SEQUENCE OF RETROVIRUS PROVIRUS RESEMBLES THAT OF BACTERIAL TRANSPOSABLE ELEMENTS
SEQUENCE OF RETROVIRUS PROVIRUS RESEMBLES THAT OF BACTERIAL TRANSPOSABLE ELEMENTS
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DOI：
10.1038/285550a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
TEMIN, HM
中科院分区：
文献类型：
--
作者：
SHIMOTOHNO, K;MIZUTANI, S;TEMIN, HM
在修饰的lambda中克隆的传染性整合型逆转录病毒[脾坏死病毒]末端区域的核苷酸序列。噬菌体克隆载体Charon 4A。病毒DNA的两端都有一个569个碱基对的直接重复序列。每一端的细胞-病毒连接包括一对5碱基对的细胞DNA直接重复序列和一对3碱基的病毒DNA反向重复序列。这种结构类似于转座元件，与逆转录病毒从细胞基因组进化而来的原始病毒假说一致。
The nucleotide sequences of the terminal regions of an infectious integrated retrovirus [spleen necrosis virus] cloned in the modified .lambda. phage cloning vector Charon 4A were elucidated. There is a 569-base pair direct repeat at both ends of the viral DNA. The cell-virus junctions at each end consist of a 5-base pair direct repeat of cell DNA next to a 3-base pair inverted repeat of viral DNA. This structure resembles that of a transposable element and is consistent with the protovirus hypothesis that retroviruses evolved from the cell genome.