Identification of the Bcl-2 family protein gene BOK from orange-spotted grouper (Epinephelus coioides) involved in SGIV infection
Identification of the Bcl-2 family protein gene BOK from orange-spotted grouper (Epinephelus coioides) involved in SGIV infection
复制标题
斜带石斑鱼参与SGIV感染的Bcl-2家族蛋白基因BOK的鉴定
DOI：
10.1016/j.fsi.2016.03.026
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发表时间：
2016
影响因子：
4.7
通讯作者：
Jian Jichang
中科院分区：
文献类型：
--
作者：
Cai Jia;Yu Dapeng;Wei Shina;Tang Jufen;Lu Yishan;Wu Zaohe;Qin Qiwei;Jian Jichang
细胞凋亡在许多生理过程和免疫应答中起着重要作用。BOK是细胞凋亡的主要调控因子之一。本研究从Epinephelus coioides中克隆了一个新的BOK同源基因（Ec-BOK）。Ec-BOK编码210个氨基酸，与stegastespartitusbok蛋白有97%的同源性，包含4个BH结构域和1个跨膜区。Ec-BOK在所有组织中广泛表达，其中肾和脾表达量较高。体外感染SGIV后，其表达水平上调。进一步分析发现，过表达Ec-BOK抑制了病毒基因的转录和病毒在鱼细胞中的复制。我们的研究结果表明，Ec-BOK可能在病毒免疫应答中发挥作用。
Apoptosis plays vital roles in many physiological process and immune response. BOK is one of the central regulators in apoptosis. In this study, a new BOK homolog (Ec-BOK) was cloned and characterized from Orange-spotted grouper, Epinephelus coioides. Ec-BOK encoded a 210 amino acid peptides which shared 97% identity toStegastespartitusBOK protein, contained four BH domains and one transmembrane region. Ec-BOK widely expressed in all analyzed tissues with the higher expressions in kidney and spleen. Its expression level was up-regulated after SGIV infectionin vitro. Further analysis revealed that overexpression of Ec-BOK inhibited viral genes transcriptions and virus replication in fish cell. Our findings suggested that Ec-BOK might play a role in the immune response against virus.