REPAIR OF HETERODUPLEX PLASMID DNA AFTER TRANSFORMATION INTO SACCHAROMYCES-CEREVISIAE
REPAIR OF HETERODUPLEX PLASMID DNA AFTER TRANSFORMATION INTO SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.6.10.3401
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发表时间：
1986-10-01
影响因子：
5.3
通讯作者：
KOLODNER, RD
中科院分区：
文献类型：
--
作者：
BISHOP, DK;KOLODNER, RD
纯化的含有8或12碱基对插入错配或AC或CT替代错配的异双质粒dna被用于转化酿酒酵母。两个插入错配相隔943个碱基对，至少55%的时间彼此独立修复。这表明修复束通常短于1千碱基。两种插入错配修复效率不同。通过对一个错配位点的修复效率进行比较，发现错配位点可以促进自身的修复，并影响相邻错配位点的修复。将含有单插入错配的两种质粒转化到酿酒酵母细胞中，其中一种质粒的修复产物对插入有轻微的偏好，而含有另一种质粒的转化子对插入没有修复偏好。
Purified heteroduplex plasmid DNAs containing 8- or 12-base-pair insertion mismatches or AC or CT substitution mismatches were used to transform Saccharomyces cerevisiae. Two insertion mismatches, separated by 943 base pairs, were repaired independently of each other at least 55% of the time. This suggested that repair tracts were frequently shorter than 1 kilobase. The two insertion mismatches were repaired with different efficiencies. Comparison of the repair efficiency of one mismatched site with or without an adjacent mismatch suggests that mismatches promote their own repair and can influence the repair of neighboring mismatches. When two different plasmids containing single-insertion mismatches were transformed into S. cerevisiae cells, a slight preference towards insertion was detected among repair products of one of the two plasmids, while no repair preference was detected among transformants with the second plasmid.