A molecular framework for plant regeneration
A molecular framework for plant regeneration
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DOI：
10.1126/science.1121790
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发表时间：
2006-01-20
期刊：
影响因子：
56.9
通讯作者：
Scheres, B
中科院分区：
文献类型：
--
作者：
Xu, J;Hofhuis, H;Scheres, B
植物和一些动物具有从成年组织中再生器官的强大能力。然而，再生的分子机制在很大程度上尚未被探索。在这里，我们研究了拟南芥根系的局部再生响应。激光诱导的损伤破坏了生长素（一种指导细胞命运的植物激素）在根尖上的流动，我们证明了由此产生的细胞命运变化需要过多、短根和稻草人转录因子。这些转录因子调节生长素外排PIN的表达和极性位置，促进膜蛋白在更新根尖中重建生长素运输。因此，利用胚胎根干细胞模式因子的再生机制首先响应并随后稳定新的激素分布。
Plants and some animals have a profound capacity to regenerate organs from adult tissues. Molecular mechanisms for regeneration have, however, been largely unexplored. Here we investigate a local regeneration response in Arabidopsis roots. Laser-induced wounding disrupts the flow of auxin-a cell-fate-instructive plant hormone-in root tips, and we demonstrate that resulting cell-fate changes require the PLETHORA, SHORTROOT, and SCARECROW transcription factors. These transcription factors regulate the expression and polar position of PIN auxin efflux-facilitating membrane proteins to reconstitute auxin transport in renewed root tips. Thus, a regeneration mechanism using embryonic root stem-cell patterning factors first responds to and subsequently stabilizes a new hormone distribution.