Feline foamy virus-mediated marker gene transfer:: Identification of essential genetic elements and influence of truncated and chimeric proteins
Feline foamy virus-mediated marker gene transfer:: Identification of essential genetic elements and influence of truncated and chimeric proteins
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DOI：
10.1016/j.virol.2005.12.022
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发表时间：
2006-04-25
期刊：
影响因子：
3.7
通讯作者：
Löchelt, M
中科院分区：
文献类型：
--
作者：
Bastone, P;Bravo, IG;Löchelt, M
由泡沫或痰状逆转录病毒衍生的逆转录病毒载体被认为是靶向基因传递和疫苗接种的有前途的工具。为了充分利用这一潜力，我们通过构建和分析一系列基于FFV的复制缺陷载体基因组，确定了猫泡沫病毒（FFV）基因组上必需的顺式作用序列。标记基因转移所必需的顺式作用序列被发现位于FFV基因组的两个位置：(i)在5'-未翻译区，靠近gag ATG； （ii）在pol基因的中心部分。两个顺式作用序列的存在及其在FFV基因组上的相对位置与类人猿和灵长类泡沫病毒中描述的功能对应元件相似，但不完全相同。(c) 2006爱思唯尔公司版权所有。
Retroviral vectors derived from foamy or spumaretroviruses are considered promising tools for targeted gene delivery and vaccination purposes. In order to fully exploit this potential, we identified essential cis-acting sequences on the feline foamy virus (FFV) genome by constructing and analyzing a series of FFV-based replication-deficient vector genomes. Cis-acting sequences essentially required for marker gene transfer were found to be localized at two sites on the FFV genome: (i) in the 5'-untranslated region and close to the gag ATG and (ii) in the central part of the pol gene. The presence of two cis-acting sequences and their relative location on the FFV genome are similar but not identical to the functionally corresponding elements described for simian and primate foamy viruses. (c) 2006 Elsevier Inc. All rights reserved.