The kinetics of non-lamellar phase formation in DOPE-Me: relevance to biomembrane fusion.
The kinetics of non-lamellar phase formation in DOPE-Me: relevance to biomembrane fusion.
复制标题
DOPE-Me 中非层状相形成的动力学：与生物膜融合的相关性。
DOI：
10.1007/s00232-003-0617-z
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Caffrey,M
中科院分区：
文献类型：
--
作者：
Cherezov,V;Siegel,DP;Shaw,W;Burgess,SW;Caffrey,M
The mechanism of the lamellar/inverted cubic (QII) phase transition is related to that of membrane fusion in lipid systems. N-Monomethylated dioleoylphosphatidylethanolamine (DOPE-Me) exhibits this transition and is commonly used to investigate the effects of exogenous substances, such as viral fusion peptides, on the mechanism of membrane fusion. We studied DOPE-Me phase behavior as a first step in evaluating the effects of membrane-spanning peptides on inverted phase formation and membrane fusion. These measurements show that: a) the onset temperatures forQIIand inverted hexagonal (HII) phase formation both are temperature scan rate-dependent; b) longer pre-incubation times at low temperature and lower temperature scan rates favor formation of theQIIphase; and c) in temperature-jump experiments between 61 and 65°C, the meta-stableHIIphase forms initially, and disappears slowly while theQIIphase develops. These observations are rationalized in the context of a mechanism for both the lamellar/non-lamellar phase transition and the related process of membrane fusion.
