Optical measurements reveal nature of intercellular coupling across ventricular wall.
Optical measurements reveal nature of intercellular coupling across ventricular wall.
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光学测量揭示了穿过心室壁的细胞间耦合的性质。
DOI：
10.1152/ajpheart.01245.2004
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Rosenbaum,DavidS
中科院分区：
文献类型：
--
作者：
Poelzing,Steven;Roth,BradleyJ;Rosenbaum,DavidS
Previously, we showed that intercellular uncoupling through gap junctions is an important mechanism for maintaining transmural heterogeneities of repolarization that are responsible for ventricular arrhythmias in disease states such as heart failure. However, rotational anisotropy between transmural muscle layers also may influence coupling. To determine the effect of rotational anisotropy on transmural coupling, we developed a numerical three-dimensional model of passive cardiac tissue in which rotational anisotropy was varied in a controlled fashion. Simulations of optical mapping demonstrated that spatial averaging produced a voltage decay in space best fit by a single decaying exponential compared with the theoretically predicted decay. As fiber orientation varied by 90° with respect to the transmural surface, the effective transmural space constant (λTM) changed by only 0.31% in simulations. In contrast, reducing intercellular conductivity by 24% decreased λTMby 7.7%. In the canine wedge preparation (n= 5), λ measured by optical mapping of the epicardial and subepicardial surface was similar transverse (λTV= 0.73 ± 0.10 mm) and transmural (λTM= 0.70 ± 0.08 mm) to subepicardial fibers. We confirmed previous findings that λTMin subepicardial layers was significantly reduced by 14 ± 2% compared with deeper layers of myocardium, providing evidence for transmural uncoupling in the epicardial-midmyocardial interface. These data establish the theoretical and experimental basis for measuring intercellular coupling between muscle layers spanning the ventricular wall with optical mapping techniques. Furthermore, this study demonstrates that transmural uncoupling at the epicardial-midmyocardial interface may be attributable to heterogeneous expression of cardiac gap junctions and not rotational anisotropy.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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DOI：
--
发表时间：
1997
期刊：
影响因子：
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作者：
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通讯作者：
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DOI：
--
发表时间：
2001
期刊：
影响因子：
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作者：
D. Rosenbaum;J. Jalife
通讯作者：
J. Jalife