Detection of MRSA ST3061-t843-mecC and ST398-t011-mecA in white stork nestlings exposed to human residues
Detection of MRSA ST3061-t843-mecC and ST398-t011-mecA in white stork nestlings exposed to human residues
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DOI：
10.1093/jac/dkv314
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发表时间：
2016-01-01
影响因子：
5.2
通讯作者：
Torres, Carmen
中科院分区：
文献类型：
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作者：
Gomez, Paula;Lozano, Carmen;Torres, Carmen
目的：本研究的目的是分析金黄色葡萄球菌/耐甲氧西林金黄色葡萄球菌在鹳气管携带的流行情况，并研究所获得的菌株的耐药性和毒力基因。金黄色葡萄球菌和MRSA恢复。药敏试验，并通过PCR分析耐药，毒力和免疫逃避簇（IEC）基因的存在。S.金黄色葡萄球菌分离株通过SPA和AGR分型表征。对mecC阳性菌株进行SCC mec分型，并对17株筛选出的S.结果：金黄色葡萄球菌（S.在32/92份样本中鉴定出金黄色葡萄球菌分离株（34.8%），并回收了38株分离株。S.填埋场雏鸟的金黄色葡萄球菌感染率（24/43，55.8%）高于自然栖息地（8/49，16.3%）。来自垃圾填埋场相关菌落的3只鸡携带MRSA，2只携带mecA阳性菌株[克隆复合体（CC）5-spa-t002和CC 398-spa-t011]，1只携带mecC阳性菌株[序列类型（ST）3061-CC 130-spat 843-agr-III-SCCmecXI）。没有MRSA分离株呈现IEC基因。共分离到35个MSSA菌株，分属于CC 5、CC 7、CC 22、CC 30、CC 45、CC 59、CC 133和CC 398等18个不同的spa类型。agr类型为Ⅰ型（63%）、Ⅱ型（29%）和Ⅲ型（8%）。在MSSA中鉴定出的抗性和毒力基因为blaZ（n = 25）、tet（T）（n = 9）、tet（A）（n = 1）、泰特（M）（n = 2）、fexA（n = 3）、str（n = 2）、tst（n = 2）、eta（n = 1）和cna（n = 15）。在MSSA分离株中发现IEC B、C、D和G型，并鉴定出2株新的ST（ST 3060和ST 3061）。金黄色葡萄球菌，包括ST 398分离株。在暴露于人类残留物的垃圾填埋场相关菌落的鹳中检测到品系CC 398-mecA和CC 130-mecC的MRSA分离株。
Objectives: The objective of this study was to analyse the prevalence of tracheal carriage of Staphylococcus aureus/MRSA in storks and to study the resistance and virulence genes in the obtained isolates.Methods: Tracheal samples from 92 stork nestlings of two landfill-associated and two natural-habitat colonies were inoculated in specific media for S. aureus and MRSA recovery. Antimicrobial susceptibility was tested, and the presence of resistance, virulence and immune evasion cluster (IEC) genes was analysed by PCR. S. aureus isolates were characterized by spa and agr typing. Staphylococcal cassette chromosome (SCC) mec type was determined for mecC-positive isolates, and MLST was performed for 17 selected S. aureus isolates.Results: S. aureus isolates were identified in 32/92 samples (34.8%), and 38 isolates were recovered. The prevalence of S. aureus was higher in nestlings from landfills (24/43, 55.8%) than in those fromnatural habitats (8/49, 16.3%). Three birds from landfill-associated colonies carried MRSA, two with mecA-positive strains [clonal complex (CC) 5-spa-t002 and CC398-spa-t011] and one with a mecC-positive strain [sequence type (ST) 3061-CC130-spat843-agr-III-SCCmecXI). None of the MRSA isolates presented IEC genes. Thirty-five MSSA isolates, which showed 18 different spa types (ascribed to CC5, CC7, CC22, CC30, CC45, CC59, CC133 and CC398), were obtained. The agr types detected were I (63%), II (29%) and III (8%). Resistance and virulence genes identified in MSSA were blaZ (n = 25), erm(T) (n = 9), erm(A) (n = 1), tet(M) (n = 2), fexA (n = 3), str (n = 2), tst (n = 2), eta (n = 1) and cna (n = 15). The IEC types B, C, D and G were found in MSSA isolates, and two new STs were identified (ST3060 and ST3061).Conclusions: White storks are frequently tracheal carriers of S. aureus, including ST398 isolates. MRSA isolates of lineages CC398-mecA and CC130-mecC were detected in storks from landfill-associated colonies exposed to human residues.