Antioxidant enzymes in malignant prostate cell lines and in primary cultured prostatic cells
Antioxidant enzymes in malignant prostate cell lines and in primary cultured prostatic cells
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DOI：
10.1016/s0891-5849(96)00613-2
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发表时间：
1997-01-01
影响因子：
7.4
通讯作者：
Loening, SA
中科院分区：
文献类型：
--
作者：
Jung, K;Seidel, B;Loening, SA
本文测定了雄激素敏感的LNCaP细胞、雄激素不敏感的PC-3和DU145细胞以及人前列腺良恶性组织培养物中过氧化氢酶、谷胱甘肽还原酶(GR)、谷胱甘肽S转移酶(GST)、谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)的活性。原代培养的人癌组织细胞与正常组织的酶活性无明显差异。三种永久性细胞系的酶活性(超氧化物歧化酶、过氧化氢酶、GR)或高于原代细胞，或低于原代细胞。LNCaP细胞的过氧化氢酶和GR显著高于PC-3和DU 145细胞，而GST显著低于PC-3和DU 145细胞。PC-3和DU 145细胞的GST和三种细胞系的超氧化物歧化酶无显著差异。三种永久性细胞系的过氧化氢酶、GPX和GR值有显著差异。前列腺癌细胞系的不同酶设备为实验测试癌症治疗的新概念提供了基础，这有助于系统地调节前列腺癌的抗氧化防御系统。(C)1997年爱思唯尔科学公司。
The antioxidant enzymes catalase, glutathione reductase(GR), glutathione S-transferase(GST), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were determined in the androgen-responsive LNCaP and androgen-nonresponsive PC-3 and DU 145 cells as well as in prostatic epithelial cell cultures of benign and malignant human prostatic tissue. There were no differences between the enzyme activities of the human primary cell cultures from cancerous tissue and their normal counterparts. The enzyme activities of the three permanent cell lines were either higher (SOD, catalase, GR) or lower (GST, GPx) than in the primary cell cultures. In LNCaP cells catalase and GR were significantly higher, GST, in contrast, was significantly lower than in PC-3 and DU 145 cells. GST in PC-3 and DU 145 cells, and SOD in all the three cell lines showed no significant differences. Catalase, GPx and GR values were significantly different in the three permanant cell lines. The different enzymatic equipment of the prostate cancer cell lines provides the basis for experimental testing of new concepts of cancer treatment with the help of systematic modulations of the antioxidant defence systems in prostate cancer. (C) 1997 Elsevier Science Inc.