Mutations affecting the development of the embryonic zebrafish brain.
Mutations affecting the development of the embryonic zebrafish brain.
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DOI：
10.5167/uzh-232
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发表时间：
1996-12
期刊：
影响因子：
4.6
通讯作者：
A. Schier;S. Neuhauss;M. Harvey;J. Malicki;L. Solnica-Krezel;D. Stainier;F. Zwartkruis;S. Abdelilah;D. Stemple;Z. Rangini;Hong Yang;W. Driever
中科院分区：
文献类型：
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作者：
A. Schier;S. Neuhauss;M. Harvey;J. Malicki;L. Solnica-Krezel;D. Stainier;F. Zwartkruis;S. Abdelilah;D. Stemple;Z. Rangini;Hong Yang;W. Driever
在斑马鱼胚胎突变体的大规模突变筛选中，我们已经确定了影响斑马鱼大脑形态发生的24个位点的63个突变。研究了标记基因的表达和轴突支架的完整性，以研究脑区域化、神经发生和轴突发生的异常。突变体大致可分为两类，一类影响沿前后轴或背腹轴的区域化，另一类影响脑形态的一般特征。第一组包括一个基因座，该基因座需要在体细胞发生开始时产生中脑-后脑边界区域的样本。四个基因座被确定影响大脑的背-腹模式，包括先前描述的独眼基因座。这类的突变胚胎显示腹侧神经外胚层结构的减少和眼睛的可变融合。第二组包括影响脑室形成的一大类突变。对这类动物的分析揭示了在胚胎发生时心室增大需要一个功能良好的心血管系统。一个位点的突变导致多余的初级神经元的形成，这种表型使人联想到果蝇的神经原性突变。这里描述的其他突变表型范围从束状和轴突的生长异常到神经管直径的缺陷。鉴定的基因座为进一步分析斑马鱼胚胎大脑的发育奠定了遗传基础。
In a large scale mutagenesis screen for embryonic mutants in zebrafish, we have identified 63 mutations in 24 loci affecting the morphogenesis of the zebrafish brain. The expression of marker genes and the integrity of the axonal scaffold have been studied to investigate abnormalities in regionalization, neurogenesis and axonogenesis in the brain. Mutants can be broadly classified into two groups, one affecting regionalization along the anterior-posterior or dorsal-ventral axis, and the other affecting general features of brain morphology. The first group includes one locus that is required to generate the anlage of the midbrain-hindbrain boundary region at the beginning of somitogenesis. Four loci were identified that affect dorsal-ventral patterning of the brain, including the previously described cyclops locus. Mutant embryos of this class show a reduction of ventral neuroectodermal structures and variable fusion of the eyes. The second group includes a large class of mutations affecting the formation of brain ventricles. Analysis of this class reveals the requirement of a functional cardiovascular system for ventricle enlargement during embryogenesis. Mutations in one locus lead to the formation of supernumerary primary neurons, a phenotype reminiscent of neurogenic mutants in Drosophila. Other mutant phenotypes described here range from abnormalities in the fasciculation and outgrowth of axons to defects in the diameter of the neural tube. The identified loci establish the genetic foundation for a further analysis of the development of the zebrafish embryonic brain.