Effect of cobalt and chromium ions on human MG-63 osteoblasts in vitro: Morphology, cytotoxicity, and oxidative stress
Effect of cobalt and chromium ions on human MG-63 osteoblasts in vitro: Morphology, cytotoxicity, and oxidative stress
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DOI：
10.1016/j.biomaterials.2006.01.035
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发表时间：
2006-06-01
期刊：
影响因子：
14
通讯作者：
Huk, OL
中科院分区：
文献类型：
--
作者：
Fleury, C;Petit, A;Huk, OL
最近的研究表明，Co2+和Cr3+离子诱导巨噬细胞死亡、tnf - α分泌和蛋白质氧化。然而，对于腐蚀产物对成骨细胞的影响知之甚少，而成骨细胞在控制骨重塑中起着至关重要的作用。本研究的目的是探讨Co2+ (0-10ppm)和Cr3+ (0- 150ppm)油对人MG-63成骨样细胞的细胞毒性和氧化应激的影响。显微镜分析显示细胞的形状、大小和数量发生了变化。Co2+对这些参数的影响大于Cr3+。细胞计数显示MG-63成骨细胞数量明显减少，且呈时间和剂量依赖性，Co2+毒性大于Cr3+。3-（4,5-二甲基噻唑-2-基）2,5-二苯基溴化四唑（MTT）分析也显示Co2+和Cr3+离子存在时细胞活性降低。氧化和硝化蛋白是氧化应激的两种标记物，它们被检测为单条带，并显示出时间和剂量依赖性的蛋白质修饰。我们还研究了三种抗氧化酶的表达。血红素加氧酶-1的表达在24h后均升高，随后逐渐降低。在Co2+和Cr3+离子作用下，谷胱甘肽过氧化物酶的表达也以浓度和时间依赖性的方式增加。Co2+降低过氧化氢酶的表达，而Cr3+增加过氧化氢酶的表达，并呈剂量和时间依赖性。总之，本研究表明，Cr-3+和Co2+对MG-63成骨细胞具有细胞毒性作用，并有可能改变其氧化还原状态。(c) 2006 Elsevier Ltd.版权所有。
Recent studies demonstrated that Co2+ and Cr3+ ions induced cell mortality, TNF-alpha secretion, and oxidation of proteins in macrophages. However, little is known about the effects of corrosion products on the osteogenic cells, which have a crucial role in controlling bone remodeling. The aim of the present study was to investigate the effect of Co2+ (0-10ppm) and Cr3+ (0-150 ppm) oil human MG-63 osteoblast-like cells in term of cytotoxicity and oxidative stress. Microscopic analysis demonstrated changes in shape, size, and number of cells. Co2+ had a greater effect on these parameters than Cr3+. Cell counting showed a significant decrease in the number of MG-63 osteoblasts in a time- and dose-dependent manner, with Co2+ more toxic than Cr3+. The 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) analysis also showed a decreased Cellular activity in presence of Co2+ and Cr3+ ions. Oxidized and nitrated proteins, two markers of oxidative stress, were detected as single bands and revealed time- and dose-dependent protein modifications. We also studied the expression of three antioxidant enzymes. The expression of heme oxygenase-1 was increased by both ions after 24h, before decreasing gradually thereafter. Glutathione peroxidase expression was also increased in a concentration- and time-dependent manner by both Co2+ and Cr3+ ions. Co2+ decreased catalase expression while Cr3+ increased it in a dose- and time-dependent manner. In conclusion, this study demonstrated that Cr-3+ and Co2+ have a cytotoxic effect oil MG-63 osteoblasts and have the potential to modify their redox state. (c) 2006 Elsevier Ltd. All rights reserved.