Oral administration of 5-hydroxytryptophan aggravated periodontitis-induced alveolar bone loss in rats
Oral administration of 5-hydroxytryptophan aggravated periodontitis-induced alveolar bone loss in rats
复制标题
口服5-羟色氨酸加重大鼠牙周炎引起的牙槽骨丢失
DOI：
10.1016/j.archoralbio.2015.01.015
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发表时间：
2015-05-01
影响因子：
3
通讯作者：
Wang, Hang
中科院分区：
文献类型：
--
作者：
Li, Xianxian;Wu, Xiangnan;Wang, Hang
目的：5-羟色胺（5-HTP）是5-羟色胺的前体，5-HTP被广泛用作膳食补充剂以提高5-羟色胺水平。血清素最近被发现是一个新的和重要的球员在骨代谢。由于外周5-羟色胺对骨的负调节作用，定期服用5-HTP可能会影响牙槽骨代谢，从而影响牙周炎引起的牙槽骨丢失。本研究旨在探讨5-羟色氨酸对牙周炎牙槽骨破坏的影响。设计：雄性SD大鼠随机分为以下四组：（1）对照组（2）5-HTP组（5-HTP，25 mg/kg/天，无结扎）;（3）L组（结扎+生理盐水安慰剂）;和（4）L + 5-HTP组（结扎+ 5-HTP，25 mg/kg/天）。通过ELISA测定血清5-羟色胺水平。牙槽骨进行了评估与微型计算机断层扫描和组织学。抗酒石酸酸性磷酸酶染色用于评估破骨细胞生成。结果：5-HTP能显著提高血清5-羟色胺（5-HT）水平，并能促进牙周组织和白细胞介素-6（IL-6）阳性骨细胞中RANKL和OPG的表达。在实验性牙周炎大鼠中，5-HTP增加牙槽骨吸收，加重牙槽骨微结构破坏。5-HTP还刺激破骨细胞生成，增加RANKL/OPG比值和IL-6阳性骨细胞数量。结论：5-HTP可加重牙周炎大鼠牙槽骨的丢失，破坏牙槽骨的微结构，提示5-HTP可加重牙周炎的严重程度。(C)2015爱思唯尔有限公司版权所有。
Objective: 5-Hydroxytryptophan (5-HTP) is the precursor of serotonin and 5-HTP has been widely used as a dietary supplement to raise serotonin level. Serotonin has recently been discovered to be a novel and important player in bone metabolism. As peripheral serotonin negatively regulates bone, the regular take of 5-HTP may affect the alveolar bone metabolism and therefore influence the alveolar bone loss induced by periodontitis. The aim of this study was to investigate the effect of 5-HTP on alveolar bone destruction in periodontitis.Design: Male Sprague-Dawley rats were randomly divided into the following four groups: (1) the control group (without ligature); (2) the 5-HTP group (5-HTP at 25 mg/kg/day without ligature); (3) the L group (ligature + saline placebo); and (4) the L + 5-HTP group (ligature + 5-HTP at 25 mg/kg/day). Serum serotonin levels were determined by ELISA. The alveolar bones were evaluated with micro-computed tomography and histology. Tartrate-resistant acid phosphatase staining was used to assess osteoclastogenesis. The receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) expression in the periodontium as well as the interleukin-6 positive osteocytes were analysed immunohistochemically.Results: 5-HTP significantly increased serum serotonin levels. In rats with experimental periodontitis, 5-HTP increased alveolar bone resorption and worsened the micro-structural destruction of the alveolar bone. 5-HTP also stimulated osteoclastogenesis and increased RANKL/OPG ratio and the number of IL-6 positive osteocytes. However, 5-HTP treatment alone did not cause alveolar bone loss in healthy rats.Conclusion: The present study showed that 5-HTP aggravated alveolar bone loss, deteriorated alveolar bone micro-structure in the presence of periodontitis, which suggests 5-HTP administration may increase the severity of periodontitis. (C) 2015 Elsevier Ltd. All rights reserved.