A DNA binding protein specific for an origin of replication of herpes simplex virus type 1.
A DNA binding protein specific for an origin of replication of herpes simplex virus type 1.
复制标题
一种对 1 型单纯疱疹病毒复制起点具有特异性的 DNA 结合蛋白。
DOI：
10.1073/pnas.83.17.6322
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发表时间：
1986
影响因子：
11.1
通讯作者：
Lehman,IR
中科院分区：
文献类型：
--
作者：
Elias,P;O'Donnell,ME;Mocarski,ES;Lehman,IR
我们已经鉴定出一种与单纯疱疹病毒1型基因组的复制起点（oris）特异性结合的蛋白质。Oris结合蛋白，仅在感染细胞的核提取物中可检测到，显示与疱疹病毒诱导的DNA聚合酶和DNA结合蛋白ICP8相同的出现时间过程。部分纯化的oris结合蛋白产生DNA酶I“足迹”，其跨越90个碱基对的最小oris序列中的18个。因此，oris结合蛋白可能类似于病毒和染色体DNA复制起始所需的其他来源特异性结合蛋白。
We have identified a protein that binds specifically to an origin of replication (oris) of the herpes simplex virus type 1 genome. The oris binding protein, detectable only in nuclear extracts of infected cells, shows the same time course of appearance as the herpesvirus-induced DNA polymerase and the DNA binding protein ICP8. The partially purified oris binding protein generates a DNase I "footprint" that spans 18- of the 90-base-pair minimal oris sequence. The oris binding protein may, therefore, be analogous to other origin-specific binding proteins that are required for the initiation of viral and chromosomal DNA replication.