Leaf Senescence of Soybean at Reproductive Stage is Associated with Induction of Autophagy-related Genes, GmATG8c, GmATG8i and GmATG4
Leaf Senescence of Soybean at Reproductive Stage is Associated with Induction of Autophagy-related Genes, GmATG8c, GmATG8i and GmATG4
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DOI：
10.1626/pps.14.141
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发表时间：
2011-04-01
影响因子：
2.5
通讯作者：
Iwaya-Inoue, Mari
中科院分区：
文献类型：
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作者：
Htwe, Nang Myint Phyu Sin;Yuasa, Takashi;Iwaya-Inoue, Mari
自噬似乎在衰老过程中起着大量蛋白质降解和N再动员的作用。大豆脱荚抑制叶片氮的再动员，导致“绿茎综合征”。分析了在生殖期不脱荚（对照）、一半脱荚（50%脱荚）和全部脱荚（100%脱荚）的大豆植株叶片和茎中自噬相关基因（ATGs）的表达和氮含量。100%脱荚后植株叶片总氮含量和SPAD保持在恒定水平，50%脱荚后植株叶片总氮含量和SPAD在脱荚后4 ~ 5周逐渐下降，而对照植株叶片总氮含量和SPAD在此期间迅速下降。GmATG8c和GmATG8i在对照叶片中表达量在4周达到峰值，50%和100%脱荚后基因表达量逐渐增加。ATGs的瞬时上调与叶片氮含量和SPAD的下降同时发生。这些结果表明，ATGs参与了叶片衰老和氮素从叶片到荚果的再运输。
Autophagy appears to function in bulk protein degradation and N remobilization in senescence. Depodding of soybean suppresses N remobilization from leaves, leading to "green stem syndrome". Expression of autophagy-related genes (ATGs) and N contents of the leaves and stem was analyzed in soybean plants from none (control), half (50% depodding) and all (100% depodding) of flowers and pods were removed at the reproductive 5 stage. Total N content and SPAD in leaves of the plant after 100% depodding were retained at a constant level and those after 50% depodding gradually decreased from 4 to 5 wk after depodding, while those in the control plants rapidly decreased during this period. Expression of GmATG8c and GmATG8i in leaves peaked at 4 wk in control while the gene expression increased gradually after 50% and 100% depodding. The transient up-regulation of ATGs and the decline of leaf N content and SPAD occurred simultaneously. These results indicated that ATGs are involved in leaf senescence and N remobilization from leaf to pod.