Quantitative variation of the common acute lymphoblastic leukemia antigen (gp100) on leukemic marrow blasts.
Quantitative variation of the common acute lymphoblastic leukemia antigen (gp100) on leukemic marrow blasts.
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白血病骨髓母细胞上常见急性淋巴细胞白血病抗原 (gp100) 的数量变化。
DOI：
10.1172/jci111368
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Murphy,SB
中科院分区：
文献类型：
--
作者：
Look,AT;Melvin,SL;Brown,LK;Dockter,ME;Roberson,PK;Murphy,SB
研究了B细胞前体急性淋巴细胞白血病（ALL）患儿骨髓原始细胞中常见ALL抗原（CALLA）定量表达的差异。在42名未经治疗的患者中，35名通过J-5单克隆抗体结合的流式细胞术（FCM）分析具有可检测量的CALLA。使用FCM技术，提供了一个给定的细胞表面抗原，细胞大小和DNA含量的相关测量，我们检测到增加CALLA表达的淋巴母细胞从G 0/G1期通过S期的细胞周期。抗原密度（每单位胚细胞表面积）在相同的时间间隔内保持相对恒定，表明这种变化不是由于S期特异性增强CALLA表达。8例超二倍体细胞DNA含量，其中7例，只有细胞克隆异常的DNA含量表达CALLA标记。每个患者的CALLA平均量在研究组内范围很广，从非常高到勉强可检测。这种变化与细胞大小、干细胞DNA含量、S期细胞百分比或细胞质免疫球蛋白的存在或不存在没有明显关系。单变量比例风险分析结果显示，S期细胞的CALLA定量水平（P = 0.048）和白色细胞计数（P = 0.012）对治疗结果均有显著贡献。对于整个CALLA+组，CALLA相对量小于中值的患者具有较高的失败率，这与原始细胞缺乏可检测的CALLA的7名HLA-DR+患者几乎相同。观察到的CALLA定量表达的患者间差异与B细胞前体分化的公认步骤一致，可能有助于区分预后较差的患者。
Marrow blasts from children with B cell precursor acute lymphoblastic leukemia (ALL) were studied for differences in quantitative expression of the common ALL antigen (CALLA). Of 42 untreated patients, 35 had detectable amounts of CALLA by flow cytometric (FCM) analysis of J-5 monoclonal antibody binding. Using an FCM technique that provides correlated measurements of a given cell surface antigen, cell size, and DNA content, we detected increased CALLA expression as lymphoblasts moved from G0/G1 phase through S phase of the cell cycle. The density of the antigen (per unit of blast surface area) remained relatively constant over the same interval, indicating that the change was not due to S phase-specific enhancement of CALLA expression. Eight cases had hyperdiploid cellular DNA content and in seven of these, only cells with clonal abnormalities of DNA content expressed the CALLA marker. Mean amounts of CALLA for each patient ranged widely within the study group, from very high to marginally detectable. This variation had no discernible relation to cell size, stem-line DNA content, percentage of cells in S phase, or the presence or absence of cytoplasmic immunoglobulin. Results of a univariate proportional hazards analysis showed that both quantitative level of CALLA for S phase cells (P = 0.048) and white blood cell count (P = 0.012) had made significant contributions to treatment outcome. Patients with relative amounts of CALLA less than the median value for the entire CALLA+ group had a higher rate of failure, which was virtually identical to that for the seven HLA-DR+ patients whose blasts lacked detectable CALLA. The observed interpatient variation in quantitative expression of CALLA is consistent with recognized steps in B cell precursor differentiation and may be useful in distinguishing patients with a less favorable prognosis.Images
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定义人类血清学上不同的 HLA-D/DR 相关 Ia 样抗原的单克隆抗体。
影响因子：
2.7
作者：
Nadler,LM;Stashenko,P;Hardy,R;Pesando,JM;Yunis,EJ;Schlossman,SF
通讯作者：
Schlossman,SF
细胞表面抗原：儿童急性淋巴细胞白血病的预后意义。
影响因子：
20.3
作者：
S. Sallan;J. Ritz;J. Pesando;R. Gelber;C. O'Brien;S. Hitchcock;F. Coral;S. Schlossman
通讯作者：
S. Schlossman
使用单克隆抗体和流式细胞术分析人黑色素瘤细胞系 HLA-A、B、Ia 样和黑色素瘤相关抗原表达的异质性。
影响因子：
11.2
作者：
Burchiel,SW;Martin,JC;Imai,K;Ferrone,S;Warner,NL
通讯作者：
Warner,NL
单克隆抗体表型分析在儿童急性淋巴细胞白血病中的临床用途
DOI：
--
发表时间：
1982
期刊：
The Lancet
影响因子：
--
作者：
J. Kersey;C. Abramson;G. Perry;A. Goldman;M. Nesbit;K. Gajl;T. Lebien
通讯作者：
T. Lebien
人淋巴细胞的细胞DNA、膜抗原和光散射的相关分析。
DOI：
--
发表时间：
2005
期刊：
Cytometry
影响因子：
--
作者：
Raul C. Braylan;Neal A. Benson;V. A. Nourse;Howard S. Kruth
通讯作者：
Howard S. Kruth