DHD4, a CONSTANS-like family transcription factor, delays heading date by affecting the formation of the FAC complex in rice
DHD4, a CONSTANS-like family transcription factor, delays heading date by affecting the formation of the FAC complex in rice
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DHD4 是一种类 CONSTANS 家族转录因子，通过影响水稻 FAC 复合体的形成来延迟抽穗期
DOI：
10.1016/j.molp.2020.11.013
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发表时间：
2021-02-01
期刊：
影响因子：
27.5
通讯作者：
Wan, Jianmin
中科院分区：
文献类型：
--
作者：
Cai, Maohong;Zhu, Shanshan;Wan, Jianmin
抽穗期（或开花期）是水稻最重要的农艺性状之一，影响其区域适应性和作物产量。许多水稻抽穗期的主效基因已被鉴定，但在实践中，由于它们对抽穗期的影响巨大，很难用于水稻分子育种。对抽穗日期影响较小的基因很少被报道，这些基因对于微调开花时间而不造成显着的产量损失更为理想。在这项研究中，我们发现了一种新的轻微影响标题日期抑制因子，延迟标题日期 4 (DHD4)。在自然长日照条件下，与野生型植物相比，dhd4 突变体表现出稍早的开花表型，但没有明显的产量损失。 DHD4 编码位于细胞核中的 CONSTANS 样转录因子。分子、生化和遗传检测表明，DHD4可以与14-3-3竞争与OsFD1相互作用，从而影响Hd3a-14-3-3-OsFD1三蛋白FAC复合物的形成，导致OsMADS14和OsMADS15表达减少，最终延迟开花。总而言之，这些结果为水稻开花时间的调控提供了新的思路，并为微调开花时间以提高水稻的区域适应性提供了有希望的目标。
Heading date (or flowering time) is one of the most important agronomic traits in rice, influencing its regional adaptability and crop yield. Many major-effect genes for rice heading date have been identified, but in practice they are difficult to be used for rice molecular breeding because of their dramatic effects on heading date. Genes with minor effects on heading date, which are more desirable for fine-tuning flowering time without significant yield penalty, were seldom reported. In this study, we identified a new minor-effect heading date repressor, Delayed Heading Date 4 (DHD4). The dhd4 mutant shows a slightly earlier flowering phenotype without a notable yield penalty compared with wild-type plants under natural long-day conditions. DHD4 encodes a CONSTANS-like transcription factor localized in the nucleus. Molecular, biochemical, and genetic assays show that DHD4 can compete with 14-3-3 to interact with OsFD1, thus affecting the formation of the Hd3a-14-3-3-OsFD1 triprotein FAC complex, resulting in reduced expression of OsMADS14 and OsMADS15, and ultimately delaying flowering. Taken together, these results shed new light on the regulation of flowering time in rice and provide a promising target for fine-tuning flowering time to improve the regional adaptability of rice.