Site-specific gene expression in vivo by direct gene transfer into the arterial wall.
Site-specific gene expression in vivo by direct gene transfer into the arterial wall.
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DOI：
10.1126/science.2119055
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发表时间：
1990-09
期刊：
影响因子：
56.9
通讯作者：
E. Nabel;G. Plautz;G. Nabel
中科院分区：
文献类型：
--
作者：
E. Nabel;G. Plautz;G. Nabel
重组β-半乳糖苷酶基因已经通过用鼠嗜中性逆转录病毒载体直接感染或通过使用脂质体的DNA转染在体内特定动脉段中表达。血管壁中的几种细胞类型被转导，包括内皮细胞和血管平滑肌细胞。逆转录病毒感染后，重组报告基因表达至少5个月，没有检测到辅助病毒。通过直接逆转录病毒感染或脂质体介导的DNA转染实现的重组基因表达仅限于感染部位，并且在肝、肺、肾和脾中不存在。这些结果表明，位点特异性基因表达可以通过体内直接基因转移来实现，并且可以应用于治疗诸如动脉粥样硬化或癌症的人类疾病。
A recombinant beta-galactosidase gene has been expressed in a specific arterial segment in vivo by direct infection with a murine amphotropic retroviral vector or by DNA transfection with the use of liposomes. Several cell types in the vessel wall were transduced, including endothelial and vascular smooth muscle cells. After retroviral infection, a recombinant reporter gene was expressed for at least 5 months, and no helper virus was detected. Recombinant gene expression achieved by direct retroviral infection or liposome-mediated DNA transfection was limited to the site of infection and was absent from liver, lung, kidney, and spleen. These results demonstrate that site-specific gene expression can be achieved by direct gene transfer in vivo and could be applied to the treatment of such human diseases as atherosclerosis or cancer.