EFFECT OF CHRONIC ANEMIA ON WOUND TENSILE STRENGHT - CORRELATION WITH BLOOD VOLUME TOTAL RED BLOOD CELL VOLUME AND PROTEINS
EFFECT OF CHRONIC ANEMIA ON WOUND TENSILE STRENGHT - CORRELATION WITH BLOOD VOLUME TOTAL RED BLOOD CELL VOLUME AND PROTEINS
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DOI：
10.1097/00000658-196608000-00009
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发表时间：
1966-01-01
影响因子：
9
通讯作者：
KETCHAM, AS
中科院分区：
文献类型：
--
作者：
BAINS, JW;CRAWFORD, DT;KETCHAM, AS
本文叙述了卷绕抗拉强度测定的材料和方法。*剖腹手术中线伤口的切除部分悬挂在两个肠夹之间，并不断增加重量。缠绕的抗拉强度是通过测量破坏缠绕所需的重量来确定的。为了尽可能保持tlıe技术标准，各组之间的伤害会有所改变。”用标记有放射性同位素铬的供体红细胞，用指示剂稀释法测定血容量。大约0.15 cc的标记红细胞被用来产生尽可能小的体积变化。经过12 - 16分钟的混合期tlıe，将动物放血以收集足够的体积来计数重复样本。用双缩脲法进行三次红细胞比容和两次血清蛋白浓度测定。血清白蛋白未单独测定，因为需要淀粉凝胶电泳分离车辙的血清蛋白部分。
Materials and MethodsThe technic for determining wound ten-sile strength has been described.* An ex-cised segment of a midline laparotomy wound is suspended between two intestinal clamps and a constantly increasing weight is applied. Wound tensile strength is determined by measuring the weight required to disrupt the wound. Wounding was altered between groups in an effort to keep tlıe tcchnic as standard as possible." Blood volume was measured by the indicator dilution method using donor red blood cells tagged with a radioactive iso-tope of chromium. Approximately 0.15 cc. of tagged red cells was used to produce as little volume change as possible. After a l2-to 16-minute mixing period tlıe animal was exsanguinated to collect a sufficient volume to count duplicate samples. Microlhematocrits were done in triplicate and serum protcin concentration was done in duplicate by the biuret method. Serum albumin was not determined separately since starch gel electrophoresis is necessary to separate the serum protein fractions of ruts.