Nanostructural analysis and textural modification of tilapia fish gelatin affected by gellan and calcium chloride addition
Nanostructural analysis and textural modification of tilapia fish gelatin affected by gellan and calcium chloride addition
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DOI：
10.1016/j.lwt.2017.07.014
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发表时间：
2017-11
期刊：
影响因子：
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通讯作者：
L. Sow;Y. Peh;Bernadette Natalia Pekerti;Caili Fu;N. Bansal;Hongshun Yang
中科院分区：
文献类型：
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作者：
L. Sow;Y. Peh;Bernadette Natalia Pekerti;Caili Fu;N. Bansal;Hongshun Yang
鱼胶（FG）比哺乳动物明胶更适合宗教人士食用。FG的一种常用改性方法是将FG与多糖混合。然而，其机制尚不清楚。结果表明，以0.1 g/100 mL结冷胶、20 mmol/L CaCl 2和6.67 g/100 mL FG（180 Bloom）配制的FG凝胶，其凝胶强度、硬度、内聚性、咀嚼性以及凝胶化温度（Tg）和熔融温度（Tm）均与市售牛肉明胶（240 Bloom）相当。通过螺旋/卷曲比和球形聚集体的测定结果也观察到了改性后的FG。改性后FG聚集体的直径由472 nm减小到249 nm，与BG（272 nm，P< 0.05）相匹配。通过原子力显微镜（AFM），在改性FG中还发现了分离的结冷胶-结冷胶纤维聚集体和相关的FG-结冷胶无定形结构的共存。球状聚集体的螺旋/线团比和直径呈负相关，其背后的机制是明胶缔合的强度。尿素加成和傅里叶变换红外光谱（FTIR）证实了氢键的参与和FG-结冷胶复合物的存在。提出了一个示意性模型。由于改性FG成功地匹配了BG的纹理特性，因此有希望用FG代替BG。
Fish gelatin (FG) is more suitable for consumption by religious people than mammalian gelatin. One common modification method of FG is mixing FG with polysaccharides. However, the mechanism is not clear. We found that FG gel containing 0.1 g/100 mL gellan, 20 mmol/L CaCl2and 6.67 g/100 mL FG (180 Bloom) matched gel strength, hardness, cohesiveness, chewiness as well as gelling temperature (Tg) and melting temperature (Tm) of commercial beef gelatin (BG) (240 Bloom). The modified FG was also observed by the result of helix/coil ratio and spherical aggregates. The modification decreased the diameter of FG's aggregates from 472 to 249 nm, which matched with BG (272 nm,P< 0.05). Co-existence of segregative gellan-gellan fibrous aggregates and associated FG-gellan amorphous structure were also identified at the modified FG by atomic force microscope (AFM). The helix/coil ratio and diameter of spherical aggregates were inversely correlated, the mechanism behind was the strength of gelatin association. The involvement of hydrogen bond and presence of FG-gellan complex have been validated by urea addition and Fourier transform infrared (FTIR) spectroscopy. A schematic model was proposed. As modified FG successfully matched the of texture properties of BG, it is promising to replace BG with FG.