T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells.
T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells.
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DOI：
10.1084/jem.155.3.734
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发表时间：
1982-03-01
期刊：
影响因子：
--
通讯作者：
Krammer PH
中科院分区：
文献类型：
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作者：
Isakson PC;Puré E;Vitetta ES;Krammer PH
在脂多糖（LPS）存在的情况下，以低细胞密度培养BALB/c B细胞6天，导致出现少量IgG空斑形成细胞（PFC）。将伴刀豆球蛋白A（Con A）诱导的同种异体反应性（AKR抗B6）长期T细胞系（PK 7.1.1a和7.1.2）或T细胞杂交瘤（FS 7 -6.18）的上清液加入LPS处理的B细胞中，导致IgG PFC显著增加（比单独用LPS处理的培养物高3- 10倍）。诱导的IgG PFC的数量不受荧光激活细胞分选仪上去除IgG携带细胞的影响，表明T细胞衍生的B细胞分化因子增强sIgG-细胞的同种型转换，而不是选择和扩增预先存在的sIgG+细胞亚群。我们还研究了在不存在或存在T细胞因子的情况下产生的IgG亚类，发现PK 7.1.1a、PK 7.1.2和FS 7 -6.18上清液选择性地增加IgG 1的产生。其他几种含有多种淋巴因子的T细胞上清液没有影响，表明PK 7.1.1a、PK 7.1.2和FS 7 -6.18系产生的因子可以在LPS存在下特异性增强培养物中IgG分泌细胞的回收。我们称之为B细胞分化因子的这些因子不同于白细胞介素1、白细胞介素2、T细胞替代因子、集落刺激因子、巨噬细胞活化因子和免疫干扰素。我们的研究结果表明，T细胞系和杂交瘤产生的可溶性因子可以显着影响B细胞产生的IG的类别和亚类。
Culturing BALB/c B cells for 6 d at low cell density in the presence of lipopolysaccharide (LPS) results in the appearance of a small number of IgG plaque-forming cells (PFC). The addition of supernatants from concanavalin A (Con A)-induced alloreactive (AKR anti-B6) long-term T cell lines (PK 7.1.1a and 7.1.2) or a T cell hybridoma (FS7-6.18) to LPS-treated B cells resulted in a marked increase in IgG PFC (3--10- fold higher than in cultures treated with LPS alone. The number of induced IgG PFC was not affected by removing IgG-bearing cells on the fluorescence-activated cell sorter, indicating that T cell-derived B cell differentiation factor enhances isotype switching of sIgG- cells, rather than selecting and expanding pre-existing subpopulations of sIgG+ cells. We also investigated the subclass of IgG produced in the absence or presence of T cell factors and found that PK 7.1.1a, PK 7.1.2, and FS7-6.18 supernatants selectively increased IgG1 production. Several other T cell supernatants containing a variety of lymphokines had no effect, suggesting that PK 7.1.1a, PK 7.1.2, and FS7-6.18 lines produce factor(s) that can specifically enhance the recovery of IgG secreting cells in culture in the presence of LPS. These factors, which we have termed B cell differentiation factors, are different from interleukin 1, interleukin 2, T cell-replacing factor, colony- stimulating factor, macrophage-activating factor, and immune interferon. Our results suggest that soluble factors produced by T cell lines and hybridomas can markedly influence both the class and subclass of Ig produced by B cells.