Influence of Nutrient Concentrations on MPN Quantification and Enrichment of Nitrate-Reducing Fe(II)-Oxidizing and Fe(III)-Reducing Bacteria from Littoral Freshwater Lake Sediments
Influence of Nutrient Concentrations on MPN Quantification and Enrichment of Nitrate-Reducing Fe(II)-Oxidizing and Fe(III)-Reducing Bacteria from Littoral Freshwater Lake Sediments
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DOI：
10.1080/01490451.2014.892765
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发表时间：
2014-03
影响因子：
2.3
通讯作者：
E. D. Melton;A. Rudolph;S. Behrens;C. Schmidt;A. Kappler
中科院分区：
文献类型：
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作者：
E. D. Melton;A. Rudolph;S. Behrens;C. Schmidt;A. Kappler
应用培养依赖性研究来量化环境中铁代谢微生物是必要的，因为到目前为止还没有通用的功能标记基因用于培养非依赖性研究。培养基组成可能因研究而异，因此，我们确定了三种不同生长培养基对湖泊沉积物中硝酸盐还原铁（II）氧化剂和乳酸或醋酸氧化铁（III）还原剂的定量（mpn）和鉴定（通过优势DGGE条带的克隆和测序）的影响：低硫酸盐淡水培养基（FWM）、无菌过滤碳酸氢盐缓冲湖水（BLW）和两者的混合物（MIX）。我们始终发现BLW中的细胞少于FWM和MIX。不同培养基中富集的微生物群落的DGGE条带模式根据e -供体和受体对聚集在一起，而不是根据所使用的培养基聚集在一起。因此，虽然培养基成分显著影响定量，从而得出生态系统中目标群体的丰度和潜在意义的结论，但通过富集培养进行的生物多样性评估受培养基的影响较小，而是受供体/受体的类型和浓度的影响。
The application of culture-dependent studies to quantify Fe-metabolizing microorganisms from the environment is a necessity, as there are so far no universal functional marker genes for application in culture-independent studies. Media composition can vary between studies, therefore, we determined the effects of three different growth media on the quantification (MPNs) and identity (via cloning and sequencing of dominant DGGE bands) of nitrate-reducing Fe(II)-oxidizers and lactate- or acetate-oxidizing Fe(III)-reducers from a lacustrine sediment: low sulphate freshwater medium (FWM), sterile filtered bicarbonate-buffered lake water (BLW) and a mixture of both (MIX). We consistently found fewer cells in the BLW than in the FWM and the MIX. The DGGE banding patterns of the microbial communities enriched in different media types clustered together according to the e− donor and acceptor couples and not according to the medium used. Thus, although the medium composition significantly influenced the quantification and thereby conclusions on the abundance and potential significance of the targeted group within the ecosystem, biodiversity assessments through enrichment cultures were less influenced by the medium, but instead were affected by the type and concentration of the e− donor/acceptor.