A Wolbachia triple-strain infection generates self-incompatibility in Aedes albopictus and transmission instability in Aedes aegypti.
A Wolbachia triple-strain infection generates self-incompatibility in Aedes albopictus and transmission instability in Aedes aegypti.
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DOI：
10.1186/s13071-018-2870-0
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发表时间：
2018-05-11
影响因子：
3.2
通讯作者：
Sinkins SP
中科院分区：
文献类型：
--
作者：
Ant TH;Sinkins SP
人工引入细胞内细菌沃尔巴克氏菌（Wolbachia pipientis）的转染有可能降低蚊子种群对登革热和基孔肯雅热等病毒的传播能力。白纹伊蚊有两种沃尔巴克氏体本地菌株，但用非本地 wMel 菌株替代它们可以阻止这两种病毒的传播。 wMel 与野生型产生的细胞质不相容性模式是双向的。预计新毒株与本地毒株的共同感染将导致更有效的群体传播能力；从双向到单向细胞质不相容（CI）模型。伊蚊中的新型加本地菌株三重感染。生成白纹伊蚊 (wAlbAwAlbBwMel)。尽管三重感染的雌性与未感染的雄性完全可以繁殖，但它们表现出自交不亲和性。对解剖组织中特定菌株的 qPCR 表明，这可能是由于三重感染雌性卵巢中的一种天然菌株 (wAlbA) 被置换所致。当三株菌株感染转移到埃及伊蚊中时，该物种中的三种菌株的传播保真度表现出出人意料的低水平。这些结果表明，沃尔巴克氏体菌株的组合可能导致共同感染相互作用，从而影响 CI 和母体传播的结果。本文的在线版本 (10.1186/s13071-018-2870-0) 包含补充材料，可供授权用户使用。
Artificially-introduced transinfections of the intracellular bacterium Wolbachia pipientis have the potential to reduce the vectorial capacity of mosquito populations for viruses such as dengue and chikungunya. Aedes albopictus has two native strains of Wolbachia, but their replacement with the non-native wMel strain blocks transmission of both viruses. The pattern of cytoplasmic incompatiiblity generated by wMel with wild-types is bidirectional. Novel-plus-native-strain co-infection is predicted to lead to a more efficient population spread capacity; from a bi-directional to a uni-directional cytoplasmic incompatibility (CI) model. A novel-plus-native-strain triple-infection in Ae. albopictus (wAlbAwAlbBwMel) was generated. Although triple-infected females were fully reproductively viable with uninfected males, they displayed self-incompatibility. qPCR of specific strains in dissected tissues suggested that this may be due to the displacement of one of the native strains (wAlbA) from the ovaries of triple-infected females. When the triple strain infection was transferred into Aedes aegypti it displayed an unexpectedly low level of transmission fidelity of the three strains in this species. These results suggest that combining Wolbachia strains can lead to co-infection interactions that can affect outcomes of CI and maternal transmission. The online version of this article (10.1186/s13071-018-2870-0) contains supplementary material, which is available to authorized users.