DETERMINATION OF AMINO ACID SEQUENCE IN OLIGOPEPTIDES BY COMPUTER INTERPRETATION OF THEIR HIGH-RESOLUTION MASS SPECTRA
DETERMINATION OF AMINO ACID SEQUENCE IN OLIGOPEPTIDES BY COMPUTER INTERPRETATION OF THEIR HIGH-RESOLUTION MASS SPECTRA
复制标题
DOI：
10.1021/ja00975a045
复制
发表时间：
1966-01-01
影响因子：
15
通讯作者：
ARSENAUL.GP
中科院分区：
文献类型：
--
作者：
BIEMANN, K;CONE, C;ARSENAUL.GP
描述了一种基于某些肽衍生物的高分辨率质谱的计算机解释的寡肽氨基酸序列测定技术。它已成功应用于含有甘氨酸、丙氨酸、丝氨酸、脯氨酸、缬氨酸、苏氨酸、亮氨酸、天冬酰胺、氨基己二酸、赖氨酸、谷氨酸、蛋氨酸、组氨酸、苯丙氨酸、酪氨酸和S-苄基半胱氨酸以及乙酰基、三氟乙酰基、氯二氟乙酰基、苯甲酰基、苯甲酰基、邻苯二甲酰基、氯邻苯二甲酰基、甲苯磺酰基和3-羟基十二烷酰基作为N-末端取代基。由于其速度、灵敏度（几微克产生可用光谱）、对污染物的相对耐受性和解释的客观性，该技术在确定蛋白质结构所需的许多序列确定过程中应该证明是有用的。寡肽中氨基酸序列的确定是一项艰巨的实验任务，在三个领域至关重要：首先，最重要的是，确定蛋白质的一级结构；其次，测定更小的非蛋白肽，例如一些抗生素和相关代谢物；第三，验证合成肽的序列（和序列纯度）。
A technique for the amino acid sequence determination of oligopeptides based on the computer interpretation of the high resolution mass spectra of certain peptide derivatives is described. It has been successfully applied to di-through pentapeptides containing glycine, alanine, serine, proline, valine, threonine, leucine, asparagine,-aminoadipic acid, lysine, glutamic acid, methionine, histidine, phenylalanine, tyrosine, and S-benzylcysteine and with acetyl, trifluoroacetyl, chlorodifluoroacetyl, benzoyl, carbobenzoxy, phthaloyl, chlorophthaloyl, tosyl, and 3-hydroxydodecanoyl as N-terminal substituents. Because of its speed, sensitivity (a few micrograms yield a usable spectrum), relative tolerance toward contaminants, and objectivity of interpretation, the technique should prove use-ful in the course of the many sequence determinations required for the determination of the structure of a protein.The determination of the amino acid sequence in oligopeptides is a formidable experimental task which is of crucial importance in three areas: first and above all, in the determination of the primary structure of proteins; second, in the determination of the much smaller nonprotein peptidessuch as some antibiotics and related metabolites; and third, to verify the sequence (and sequential purity) of a synthetic peptide.