Nosema ceranae, a new microsporidian parasite in honeybees in Europe
Nosema ceranae, a new microsporidian parasite in honeybees in Europe
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DOI：
10.1016/j.jip.2006.02.005
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发表时间：
2006-06-01
影响因子：
3.4
通讯作者：
Meana, A
中科院分区：
文献类型：
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作者：
Higes, M;Martín, R;Meana, A
选择来自西班牙不同地区的 12 份成年蜜蜂样本进行分子诊断，这些样本来自具有明显种群减少迹象的蜂群，光学显微镜检查显示微孢子虫孢子呈阳性（分析的阳性样本总数的 1%）。开发了针对微孢子虫 16S rRNA 基因区域的 PCR 特异性引物，并对 PCR 产物进行了测序并与 GenBank 条目进行比较。 12个样品中有11个的测序产物与相应的蜜蜂微孢子虫序列相同。这是欧洲蜜蜂群体中首次报道中华蜜蜂。由于感染与临床疾病症状之间存在联系，因此有必要研究中华蜜蜂在欧洲蜜蜂品种中的毒力。 (c) 2006 Elsevier Inc. 保留所有权利。
Twelve samples of adult honey bees from different regions of Spain from colonies with clear signs of population depletion, positive to microsporidian spores using light microscopy (1% of total positive samples analysed), were selected for molecular diagnosis. PCR specific primers for a region of the 16S rRNA gene of Microsporidia were developed and the PCR products were sequenced and compared to GenBank entries. The sequenced products of 11 out of the 12 samples were identical to the corresponding Nosema ceranae sequence. This is the first report of N. ceranae in colonies of Apis mellifera in Europe. The suggested link of the infections to clinical disease symptoms makes imperative a study of the virulence of N. ceranae in European races of honey bees. (c) 2006 Elsevier Inc. All rights reserved.