Increased osteoblast adhesion on nanograined Ti modified with KRSR
Increased osteoblast adhesion on nanograined Ti modified with KRSR
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DOI：
10.1002/jbm.a.30954
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发表时间：
2007-03-01
影响因子：
4.9
通讯作者：
Webster, Thomas J.
中科院分区：
文献类型：
--
作者：
Balasundaram, Ganesan;Webster, Thomas J.
肽序列，如赖氨酸-精氨酸-丝氨酸-精氨酸（KRSR）选择性结合成骨细胞（骨形成细胞）的跨膜蛋白聚糖（如硫酸肝素），因此，正在积极研究骨科应用。此外，纳米材料（或颗粒尺寸小于100纳米的材料）是一种很有前途的新材料，与传统材料（即微米颗粒或颗粒尺寸）相比，它更能促进新骨的生长。为了结合上述两种有前途的方法来改善骨科植入物，本体外研究的目的是用KRSR肽功能化钛（Ti）表面（纳米期和常规），并研究它们的成骨细胞粘附性能。采用x射线光电子能谱、扫描电镜和原子力显微镜对材料进行表征。该体外研究结果表明，与常规钛相比，无论是否被KRSR功能化，成骨细胞在纳米期的粘附都有所增加。结果进一步表明，与未功能化的Ti和用阴性对照肽KSRR功能化的Ti相比，KRSR固定在Ti（纳米期和常规）上增加了成骨细胞的粘附。最重要的是，与KRSR功能化的常规钛相比，成骨细胞对非功能化纳米期钛的粘附增加。此外，在本研究中，观察到在任何类型的纳米期和常规钛的颗粒边界上都发生了选择性的初始成骨细胞粘附。综上所述，研究结果表明，为了改善骨科应用，不仅需要进一步研究非功能化纳米相Ti，还需要进一步研究具有KRSR功能化的纳米相Ti。(c) 2006 Wiley期刊公司
Peptide sequences such as lysine-arginine-serine-arginine (KRSR) selectively bind transmembrane proteoglycans (e.g. heparin sulfate) of osteoblasts (bone-forming cells) and are, therefore, actively being investigated for orthopedic applications. Further, nanophase materials (or materials with grain or particle sizes less than 100 nm) are promising new materials that promote new bone growth more than compared to conventional (that is, micron grain or particle size) materials. To combine the above two promising approaches for improving orthopedic implants, the objective of this in vitro study was to functionalize titanium (Ti) surfaces (both nanophase and conventional) with KRSR peptides and study their osteoblast cell adhesive properties. Materials were characterized by X-ray photoelectron spectroscopy, scanning electron microscopy, and atomic force microscopy. Results of this in vitro study provided evidence of increased osteoblast adhesion on nanophase compared to conventional Ti whether functionalized with KRSR or not. Results further showed that the immobilization of KRSR onto Ti (both nanophase and conventional) increased osteoblast adhesion compared to respective nonfunctionalized Ti and those functionalized with the negative control peptide KSRR. Most importantly, osteoblast adhesion on nonfunctionalized nanophase Ti increased compared to conventional Ti functionalized with KRSR. Further, select initial osteoblast adhesion was observed to occur at particle boundaries for any type of nanophase and conventional Ti formulated in this study. In summary, results provided evidence that not only should nonfunctionalized nanophase Ti be further studied for improved orthopedic applications but so should nanophase Ti functionalized with KRSR. (c) 2006 Wiley Periodicals, Inc.