Effects of postnatal ethanol exposure on glial cell development in rat optic nerve.
Effects of postnatal ethanol exposure on glial cell development in rat optic nerve.
复制标题
出生后乙醇暴露对大鼠视神经胶质细胞发育的影响。
DOI：
10.1016/0014-4886(90)90066-2
复制
发表时间：
1990
影响因子：
5.3
通讯作者：
Krueger,SK
中科院分区：
文献类型：
--
作者：
Phillips,DE;Krueger,SK
本研究从形态学上评价了出生后有限的酒精暴露对大鼠视神经胶质细胞发育的影响。在第5-18天，通过慢性胃造口术管人工饲养大鼠幼仔，补充牛奶饲料。实验动物在第5-9天在其饮食中接受4%乙醇，否则实验动物和对照动物接受相同的饮食。在第10、16、22、29和90天制备视神经组织用于电子显微镜检查。乙醇暴露动物在第10天和第16天每个横截面的神经胶质细胞较少，视神经横截面积较小。酒精引起的少突胶质细胞在10天的成熟延迟，证明了在存在的少突胶质细胞的总数减少，并在少突胶质细胞谱系内的未成熟细胞的出现延迟。所有这些影响都在以后的年龄得到了补偿。没有证据表明酒精诱导的神经胶质细胞或其细胞器变性。因此，出生后的酒精暴露导致少突胶质细胞成熟延迟，但似乎没有长期影响大鼠视神经的胶质细胞群。
This study morphologically evaluated the effects of limited postnatal alcohol exposure on the development of glial cells in the rat optic nerve. Rat pups were artificially reared on Days 5–18 with a supplemented milk diet fed via a chronic gastrostomy tube. Experimental animals received 4% ethanol in their diet on Days 5–9, otherwise the experimental and control animals received identical diets. Optic nerve tissues were prepared for electron microscopy on Days 10, 16, 22, 29, and 90. There were fewer glial cells per cross section and the cross-sectional areas of optic nerves were smaller on Days 10 and 16 in the ethanol-exposed animals. The alcohol caused a delay in the maturation of oligodendroglial cells at 10 days as evidenced by decreases in the total number of oligodendroglia present and by a delay in the appearance of immature cells within the oligodendroglial lineage. All of these effects were compensated for at later ages. There was no evidence of alcohol-induced degeneration of glial cells or their organelles. Thus, postnatal alcohol exposure causes a delay in oligodendrocyte maturation but appears to have no long-term effects on the glial cell population of rat optic nerve.