Direct detection of genomic DNA by enzymatically amplified SPR imaging measurements of RNA microarrays
Direct detection of genomic DNA by enzymatically amplified SPR imaging measurements of RNA microarrays
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DOI：
10.1021/ja039823p
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发表时间：
2004-04-07
影响因子：
15
通讯作者：
Corn, RM
中科院分区：
文献类型：
--
作者：
Goodrich, TT;Lee, HJ;Corn, RM
一种新的表面酶促反应方案，扩大了RNA微阵列对互补DNA结合的光学反应，用于直接检测和分析基因组DNA。RNase H可以选择性地、反复地破坏金表面DNA - RNA异质双工体中的RNA；当与表面等离子体共振（SPR）成像的无标记技术结合使用时，DNA寡核苷酸可以在1fm的浓度下检测到。这种酶扩增的SPR成像方法随后被用于检测和鉴定人类基因组DNA中TSPY基因的存在，而无需PCR扩增。
A novel surface enzymatic reaction scheme that amplifies the optical response of RNA microarrays to the binding of complementary DNA is developed for the direct detection and analysis of genomic DNA. The enzyme RNase H is shown to selectively and repeatedly destroy RNA from DNA−RNA heteroduplexes on gold surfaces; when used in conjunction with the label-free technique of surface plasmon resonance (SPR) imaging, DNA oligonucleotides can be detected at a concentration of 1 fM. This enzymatically amplified SPR imaging methodology is then utilized to detect and identify the presence of the TSPY gene in human genomic DNA without PCR amplification.