Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C
Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C
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DOI：
10.1186/1743-422x-8-214
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发表时间：
2011-05-10
期刊：
影响因子：
4.8
通讯作者：
Chen, Xiaoyue
中科院分区：
文献类型：
--
作者：
Lian, Bei;Cheng, Anchun;Chen, Xiaoyue
背景：研究表达鸭瘟病毒（DPV）糖蛋白C（gC）的DNA疫苗对鸭的免疫效果。结果：经肌肉注射和基因枪轰击两种途径免疫后，均产生了特异性的血清抗体和T细胞应答。肌肉注射免疫鸭诱导了较强的体液免疫应答，但较弱的细胞免疫应答。相比之下，一个更好的细胞介导的免疫反应，实现了通过使用基因枪将DNA包被的金珠的表皮与少至6亩的DNA.Conclusions：这表明，这两种途径的DNA接种可以用于引发病毒特异性免疫反应。虽然含DPV gC的DNA疫苗在肌肉注射和基因枪轰击中均有效，但后者可诱导更高的细胞介导的抗DPV应答。
Background: A DNA vaccine expressing glycoprotein C (gC) of duck plague virus (DPV) was evaluated for inducing immunity in ducks. The plasmid encoding gC of DPV was administered via intramuscular (IM) injection and gene gun bombardment.Results: After immunization by both routes virus-specific serum antibody and T-cell responses developed. Vaccination of ducks by IM injection induced a stronger humoral, but weaker cell-mediated immune response. In contrast, a better cell-mediated immune response was achieved by using a gene gun to deliver DNA-coated gold beads to the epidermis with as little as 6 mu g of DNA.Conclusions: This demonstrated that both routes of DNA inoculation can be used for eliciting virus-specific immune responses. Although DNA vaccine containing DPV gC is effective in both intramuscular injection and gene gun bombardment, the latter could induce significantly higher cell-mediated responses against DPV.