Measuring corticotropin-releasing hormone in pregnant women - comparing blood collection protocols for epidemiological studies
Measuring corticotropin-releasing hormone in pregnant women - comparing blood collection protocols for epidemiological studies
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DOI：
10.1111/j.1365-3016.2006.00689.x
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Rich-Edwards, JW
中科院分区：
文献类型：
--
作者：
Strong, EF;Kleinman, KP;Rich-Edwards, JW
我们描述了一个试点研究，以确定在模拟现场条件下测量促肾上腺皮质激素释放激素（CRH）水平的血液样本处理延迟的影响。CRH是一种由胎盘分泌到母体血液中的肽，在妊娠期的流行病学研究中具有重要意义。许多研究者怀疑CRH可能在采集后迅速分解，并认为CRH血样的最佳处理必须包括在冷藏条件下立即处理和血浆快速冷冻。流行病学研究往往有后勤限制，使这种快速处理不可行。为了研究血液样本处理的延迟如何影响CRH的测量水平，我们收集了33名在波士顿产前诊所就诊的孕妇的全血样本。我们比较了三种不同处理延迟后测量的CRH水平与血液采集后立即处理的样本水平，即“金标准”。延迟策略包括在处理前将新鲜采集的全血置于冷却器或冰箱中长达22小时。延迟处理与金标准处理的相关系数超过0.96。这些结果表明，CRH可以在血液样本中进行测量，这些血液样本在血液采集后旋转并冷冻长达22小时。
We describe a pilot study to determine the effect of delays in blood sample processing under simulated field conditions on measurement of corticotropin-releasing hormone (CRH) levels in pregnant women. CRH, a peptide secreted by the placenta into the maternal blood, is of interest in epidemiological studies of gestational duration. Many investigators suspected that CRH might break down quickly after collection, and believed the optimal treatment of blood samples for CRH must include immediate processing under chilled conditions and quick freezing of plasma. Epidemiological studies often have logistical constraints that make such rapid processing unfeasible. To examine how delays in the processing of blood samples might affect the level of measured CRH, we collected whole blood samples from 33 pregnant women attending a prenatal clinic in Boston. We compared CRH levels measured following three different processing delays with the levels of samples that were processed immediately after blood collection, the 'gold standard'. The delayed strategies involved placing the freshly collected whole blood in a cooler or refrigerator for up to 22 h prior to processing. Correlation coefficients comparing delayed with gold standard processing exceeded 0.96. These results suggest that CRH may be measured in blood samples that were spun and frozen up to 22 h after blood collection.