Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo
Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo
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DOI：
10.1006/bbrc.2001.6270
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发表时间：
2002-01-18
影响因子：
3.1
通讯作者：
Guilak, F
中科院分区：
文献类型：
--
作者：
Erickson, GR;Gimble, JM;Guilak, F
关节软骨几乎没有表现出内在的修复能力，并且正在开发新的组织工程方法来利用细胞疗法促进软骨再生。本研究的目的是检查脂肪组织来源的基质细胞的软骨形成潜力。基质细胞从通过吸脂获得的人皮下脂肪组织中分离出来，并在有或没有软骨形成培养基的藻酸盐培养物中在体外扩增和生长。脂肪来源的基质细胞大量合成软骨基质分子，包括 II 型、VI 型胶原蛋白和 4-硫酸软骨素。藻酸盐细胞构建体在体外软骨形成培养基中生长2周，然后皮下植入裸鼠体内4周和12周。这些样品的免疫组织化学分析显示软骨基质分子的大量产生。这些发现证明了脂肪组织来源的基质细胞在体外和体内模型中产生特征性软骨基质分子的能力，并表明这些细胞在软骨组织工程中的潜力。 (C) 2002 年爱思唯尔科学。
Articular cartilage exhibits little intrinsic repair capacity, and new tissue engineering approaches are being developed to promote cartilage regeneration using cellular therapies. The goal of this study was to examine the chondrogenic potential of adipose tissue-derived stromal cells. Stromal cells were isolated from human subcutaneous adipose tissue obtained by liposuction and were expanded and grown in vitro with or without chondrogenic media in alginate culture. Adipose-derived stromal cells abundantly synthesized cartilage matrix molecules including collagen type II, VI, and chondroitin 4-sulfate. Alginate cell constructs grown in chondrogenic media for 2 weeks in vitro were then implanted subcutaneously in nude mice for 4 and 12 weeks. Immunohistochemical analysis of these samples showed significant production of cartilage matrix molecules. These findings document the ability of adipose tissue-derived stromal cells to produce characteristic cartilage matrix molecules in both in vitro and in vivo models, and suggest the potential of these cells in cartilage tissue engineering. (C) 2002 Elsevier Science.