Specific Antibodies against Synthetic Oligopeptides of Eel GTH/TSH Subunits
Specific Antibodies against Synthetic Oligopeptides of Eel GTH/TSH Subunits
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抗 Eel GTH/TSH 亚基合成寡肽的特异性抗体
DOI：
10.2331/fishsci.65.871
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发表时间：
1999
影响因子：
1.9
通讯作者：
K. Yamauchi
中科院分区：
文献类型：
--
作者：
T. Ikeuchi;M. Nagae;P. Lokman;S. Adachi;K. Yamauchi
用人工合成的寡肽作为抗原，制备了针对日本鳗鲡促性腺激素11 α（GTH）、促甲状腺激素α（TSH）和共同α（GTH/TSH）亚基的特异性抗体，以检测GTH和TSH的合成。Western blot和免疫组化检测抗体的特异性。对鳗鱼垂体进行Western blot检测，每个抗体均检测到一条特异性条带.免疫组化结果也表明，这些抗体可以可靠地鉴别鳗鱼中的GTH、TSH和GTH β U。由于尚未公布关于鳗鱼GTH阳性细胞鉴定的细节，我们试图通过从整个GTH/TSH阳性细胞群体中减去GTH阳性细胞和TSH阳性细胞来鉴定这些细胞。在大麻哈鱼中，既不与抗大麻哈鱼GTH抑制剂反应也不与抗人TSH抑制剂反应的抗GTH/TSH α阳性细胞（大麻哈鱼-在早期卵黄形成的日本鳗鱼和中期卵黄形成的新西兰长鳍鳗鱼从野生，许多卵黄-这些结果表明，日本鳗鲡的GTH-Ⅰ ~+细胞可能是GTH-Ⅰ细胞。这些抗体的开发和验证将是有意义的，为未来的研究中的作用，GTH和TSH在日本鳗鱼。
Specific antibodies were raised against Japanese eel gonadotropic hormone 11ƒÀ(GTH ‡UƒÀ), thyroid-stimulating hormone ƒÀ (TSHƒÀ), and common a (GTH/TSHƒ¿) subunits using synthetic oligopeptides as antigens in order to examine GTH and TSH synthesis. The specificity of the antibodies was evaluated by Western blot and immunohistochemistry. By Western blot of eel pituitary, each an tibody detected one specific band. The immunohistochemical results also indicate that GTH ‡U and TSH can be reliably identified by these antibodies in eel. As details on the identify of eel GTH IƒÀ have not been published, we attempted to identify these cells by subtraction of GTH ‡UƒÀ and TSHƒÀ cells from the entire population of GTH/TSHƒ¿ positive cells. In chum salmon, the anti-GTH/TSHa posi tive cells which reacted with neither anti-salmon GTH ‡UƒÀ nor anti-human TSHƒÀ (ƒÀ --ƒ¿+ cells) proved to be anti-salmon GTH IƒÀ positive in an adjacent section. In early vitellogenic Japanese eels and mid vitellogenic New Zealand longfinned eels from the wild, many ƒÀ --ƒ¿+ cells were observed. These results suggest that the ƒÀ --ƒ¿+ cells in Japanese eels are probably GTH I cells. The development and validation of these antibodies will be significant for future studies on the role of GTHs and TSH in Japanese eels.
影响因子：
2.7
作者：
Penny Swanson;Penny Swanson;W. Dickhoff;Aubrey Gorbman;Aubrey Gorbman
通讯作者：
Penny Swanson;Penny Swanson;W. Dickhoff;Aubrey Gorbman;Aubrey Gorbman
促性腺激素的结构-功能关系。
DOI：
--
发表时间：
1987
期刊：
Recent progress in hormone research
影响因子：
--
作者：
Ryan,RJ;Keutmann,HT;Charlesworth,MC;McCormick,DJ;Milius,RP;Calvo,FO;Vutyavanich,T
通讯作者：
Vutyavanich,T