Orbital fibroblast chemokine modulation: effects of dexamethasone and cyclosporin A
Orbital fibroblast chemokine modulation: effects of dexamethasone and cyclosporin A
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DOI：
10.1136/bjo.82.3.318
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发表时间：
1998-03-01
影响因子：
4.1
通讯作者：
Elner, VM
中科院分区：
文献类型：
--
作者：
Burnstine, MA;Elner, SG;Elner, VM
眼眶炎症很常见，但眼眶组织白细胞浸润的机制尚不清楚。当暴露于促炎细胞因子时，人眼眶成纤维细胞 (OF) 表达趋化因子、白细胞介素 8 (IL-8) 和单核细胞趋化蛋白 1 (MCP-1)。检测地塞米松（DEX）和环孢素A（CSA）对OF IL-8和MCP-1的影响。方法-将培养的人OF与重组白细胞介素1β（rIL-1β；0.2、2.0、20ng/ml）单独孵育或与rIL-1β和DEX（10（-8）、10（-7）、10（-6）M）一起孵育或 CSA（3、30、300 ng/ml）24 小时。进行 ELISA 和 Northern blot 分析，分别测定 OF IL-8 和 MCP-1 蛋白分泌和 mRNA 表达。 结果-OF 缺乏组成型 IL-8 或 MCP-1 表达，但分泌大量这些趋化因子，并在 rIL-1 beta 刺激后表达两种趋化因子的大量稳态 mRNA。 DEX 对 IL-1 诱导的 IL-8 产生剂量依赖性抑制 (p
Aim-Orbital inflammation is common, but the mechanisms underlying leucocytic infiltration of orbital tissue are poorly understood. Human orbital fibroblasts (OF) express chemokines, interleukin 8 (IL-8) and monocyte chemotactic protein 1 (MCP-1), when exposed to proinflammatory cytokines. The effects of dexamethasone (DEX) and cyclosporin A (CSA) on OF IL-8 and MCP-1 were examined.Methods-Cultured human OF were incubated with recombinant interleukin 1 beta (rIL-1 beta; 0.2, 2.0, 20 ng/ml) alone or incubated with rIL-1 beta and DEX (10(-8), 10(-7), 10(-6) M) or CSA (3, 30, 300 ng/ml) for 24 hours. ELISA and northern blot analyses were performed to determine OF IL-8 and MCP-1 protein secretion and mRNA expression, respectively.Results-OF lacked constitutive IL-8 or MCP-1 expression, but secreted significant amounts of these chemokines and expressed substantial steady state mRNA for both chemokines upon rIL-1 beta stimulation. DEX caused dose dependent inhibition of IL-1 induced IL-8 (p