REPLICATION PRIMING AND TRANSCRIPTION INITIATE FROM PRECISELY THE SAME SITE IN MOUSE MITOCHONDRIAL-DNA
REPLICATION PRIMING AND TRANSCRIPTION INITIATE FROM PRECISELY THE SAME SITE IN MOUSE MITOCHONDRIAL-DNA
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DOI：
10.1002/j.1460-2075.1985.tb03817.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
CLAYTON, DA
中科院分区：
文献类型：
--
作者：
CHANG, DD;HAUSWIRTH, WW;CLAYTON, DA
哺乳动物线粒体 DNA 维持着一个新的置换环区域，其中包含转录起始的主要位点和重链 DNA 复制的起点。由于新生小鼠置换环链 5'' 末端的精确图谱位置是已知的，因此可以直接检查复制启动和转录之间的潜在关系。对与置换环区域互补的体内核酸的分析揭示了 2 spp。相同的 5'' 末端位于地图位置 16,183。一种完全是RNA，另一种是与DNA共价连接的RNA。在后者中，从RNA到DNA的转变是急剧的，发生在转录起始位点下游74-163个核苷酸的一系列先前鉴定的保守序列附近或内部。复制启动和转录的初始事件是相同的，合成 DNA 或 RNA 的决定是在短、保守的置换环模板序列控制下的下游事件。
Mammalian mitochondrial DNA maintains a novel displacement-loop region containing the major sites of transcriptional initiation and the origin of heavy strand DNA replication. Because the exact map positions of the 5'' termini of nascent mouse displacement-loop strands are known, it is possible to examine directly a potential relationship between replication priming and transcription. Analyses of in vivo nuclei acids complementary to the displacement-loop region reveal 2 spp. with identical 5'' ends at map position 16,183. One is entirely RNA and the other is RNA covalently linked to DNA. In the latter the transition from RNA to DNA is sharp, occurring near or within a series of previously identified conserved sequences 74-163 nucleotides donwnstream from the transcriptional initiation site. The initial events in replication priming and transcription are the same and that the decision to synthesize DNA or RNA is a downstream event under the control of short, conserved displacement-loop template sequences.