In vivo biocompatibilty and degradation behavior of elastic poly(L-lactide-co-ε-caprolactone) scaffolds
In vivo biocompatibilty and degradation behavior of elastic poly(L-lactide-co-ε-caprolactone) scaffolds
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DOI：
10.1016/j.biomaterials.2004.01.057
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发表时间：
2004-12-01
期刊：
影响因子：
14
通讯作者：
Kim, YH
中科院分区：
文献类型：
--
作者：
Jeong, SI;Kim, BS;Kim, YH
管状支架由非常有弹性的聚（L-丙交酯-共-ε-己内酯）（PLCL，50：50）制成。将支架与平滑肌细胞（SMC）接种并植入裸鼠体内，以研究支架的组织相容性和体内降解行为。采用苏木精-伊红染色、Masson三色染色、SM α-肌动蛋白抗体和CM-DiI标记对所有植入物进行组织学检查，证实了种植的SMC的规则形态和生物功能以及PLCL支架中血管平滑肌基质的表达。植入的PLCL支架显示出缓慢的降解时间，其中己内酯单元比丙交酯降解得更快。这可以解释的事实，即主要由CL部分组成的非晶区域降解早于硬域，其中大部分的LA单元位于。从这些结果中，在本研究中应用的支架被发现表现出良好的组织相容性，平滑肌细胞和可能是非常有用的血管组织工程。(C)2004爱思唯尔有限公司保留所有权利。
Tubular scaffolds were fabricated from very elastic poly(L-lactide-co-epsilon-caprolactone) (PLCL, 50:50). The scaffolds were seeded with smooth muscle cells (SMCs) and implanted in nude mice to investigate the tissue compatibility and in vivo degradation behavior. Histological examination of all the implants with haematoxylin and eosin staining, masson trichrome staining, SM alpha-actin antibody, and CM-DiI labeling confirmed that the regular morphology and biofunction of the SMCs seeded and the expression of the vascular smooth muscle matrices in PLCL scaffolds. The implanted PLCL scaffolds displayed a slow degradation on time, where caprolactone units were faster degraded than lactide did. This could be explained by the fact that amorphous regions composed of mainly CL moieties degraded earlier than hard domains where most of the LA units were located. From these results, the scaffolds applied in this study were found to exhibit excellent tissue compatibility to SMCs and might be very useful for vascular tissue engineering. (C) 2004 Elsevier Ltd. All rights reserved.