Rhein lysinate decreases inflammation and adipose infiltration in KK/HlJ diabetic mice with non-alcoholic fatty liver disease
Rhein lysinate decreases inflammation and adipose infiltration in KK/HlJ diabetic mice with non-alcoholic fatty liver disease
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大黄酸赖氨酸可减少患有非酒精性脂肪肝疾病的 KK/HlJ 糖尿病小鼠的炎症和脂肪浸润。
DOI：
10.1007/s12272-016-0770-4
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发表时间：
2016-07-01
影响因子：
6.7
通讯作者：
Lin, Ya-Jun
中科院分区：
文献类型：
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作者：
Wei, Jie;Zhen, Yong-Zhan;Lin, Ya-Jun
本研究旨在探讨赖氨大黄酸（RHL）对肝脏的保护作用。将小鼠分为四组：C57 BL/J对照组、KK/HlJ糖尿病模型组和25和50 mg/kg/天RHL治疗的KK/HlJ组。采用链脲佐菌素（STZ）腹腔注射和糖尿病饲料喂养的方法建立KK/HlJ糖尿病小鼠模型。在16周时，处死小鼠并收获其肝脏。结果表明，与C57 BL/J对照组相比，KK/H1 J糖尿病小鼠的体重、肝重和肝重/体重比均增加，而RHL治疗后这些值均降低。与C57 BL/J对照组相比，KK/H1 J糖尿病小鼠肝脏SOD和GSH-px水平显著降低，MDA水平显著升高。然而，这些影响被RHL改善。在KK/HlJ小鼠中观察到肝脏脂肪浸润，但在C57 BL/J小鼠中未观察到。RHL可降低肝脏脂肪浸润的发生率，并显著降低肝脏中TNF-α、IL-6、NF-κ B、SREBP-1 c和Fas的表达，以及NF-κ B的磷酸化。结论：RHL可通过减少肝脏脂肪浸润和炎症因子的表达来改善肝功能。
The objective of this study was to investigate the protective effects of rhein lysinate (RHL) on the liver. Mice were divided into four groups: C57BL/J control, the KK/HlJ diabetic model, and 25 and 50 mg/kg/day RHL-treated KK/HlJ groups. The KK/HlJ diabetic mouse model was made by injecting STZ and feeding mice diabetic food. At 16 weeks, mice were sacrificed and their livers were harvested. The results indicated that compared with the C57BL/J control group, the body weights, liver weights and liver weight-to-body weight ratio were increased in KK/HlJ diabetic mice; however, these values were decreased following treatment with RHL. Compared with the C57BL/J control, KK/HlJ diabetic mice had a significantly lower level of SOD and GSH-px in their livers, but had a significantly higher level of MDA. However, these effects were ameliorated by RHL. Hepatic adipose infiltration was observed in KK/HlJ mice, but not in C57BL/J mice. RHL decreased the incidence of hepatic adipose infiltration and significantly decreased the expression of TNF-alpha, IL-6, NF-kappa B, SREBP-1c, and Fas, as well as the phosphorylation of NF-kappa B in the liver. In conclusion, RHL can improve hepatic function by decreasing hepatic adipose infiltration and the expression of inflammatory factors.