Matrix assisted laser desorption ionization mass spectrometry imaging identifies markers of ageing and osteoarthritic cartilage.
Matrix assisted laser desorption ionization mass spectrometry imaging identifies markers of ageing and osteoarthritic cartilage.
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DOI：
10.1186/ar4560
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发表时间：
2014-05-09
影响因子：
4.9
通讯作者：
Heeren RM
中科院分区：
文献类型：
--
作者：
Peffers MJ;Cillero-Pastor B;Eijkel GB;Clegg PD;Heeren RM
软骨蛋白的分布以及软骨老化和疾病发生的变化对于了解软骨老化的过程和与年龄相关的疾病(如骨关节炎)至关重要。本研究的目的是用基质辅助激光解吸电离质谱仪(MALDI-MSI)研究衰老和骨关节炎(OA)软骨切片中的多肽组成。用MALDI Synapt™HDMS系统对幼年、老年和OA马的软骨切片进行胰酶消化和MALDI-MSI后，比较了青年、老年和OA马软骨中蛋白质的分布。在多变量分析之后，使用数据库搜索进行蛋白质鉴定。用Biomap软件对年轻软骨、老化软骨和骨关节炎软骨的多肽强度差异进行成像。对一种软骨蛋白多糖粗提物的聚集聚糖酶特异性裂解模式的分析被用来验证所识别的多肽强度的一些差异。免疫组织化学研究证实了蛋白质丰度的差异。用判别分析的方法对年轻、老年和骨关节炎的马软骨的多肽特征进行了区分。鉴定和定位了包括聚集素核心蛋白、纤维调节蛋白和软骨低聚基质蛋白在内的蛋白质。纤维连接蛋白多肽在骨性关节炎软骨中有较强的表达。鉴定了胶原素-43和软骨低聚基质蛋白的年龄特异性蛋白标记物。此外，还检测到了针对OA降解的潜在的纤维调制蛋白和二聚糖肽。MALDI-MSI为研究软骨衰老和疾病提供了一个新的平台，使得软骨中的年龄和疾病特异性多肽能够被阐明和空间解析。
Cartilage protein distribution and the changes that occur in cartilage ageing and disease are essential in understanding the process of cartilage ageing and age related diseases such as osteoarthritis. The aim of this study was to investigate the peptide profiles in ageing and osteoarthritic (OA) cartilage sections using matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). The distribution of proteins in young, old and OA equine cartilage was compared following tryptic digestion of cartilage slices and MALDI-MSI undertaken with a MALDI SYNAPT™ HDMS system. Protein identification was undertaken using database searches following multivariate analysis. Peptide intensity differences between young, ageing and OA cartilage were imaged with Biomap software. Analysis of aggrecanase specific cleavage patterns of a crude cartilage proteoglycan extract were used to validate some of the differences in peptide intensity identified. Immunohistochemistry studies validated the differences in protein abundance. Young, old and OA equine cartilage was discriminated based on their peptide signature using discriminant analysis. Proteins including aggrecan core protein, fibromodulin, and cartilage oligomeric matrix protein were identified and localised. Fibronectin peptides displayed a stronger intensity in OA cartilage. Age-specific protein markers for collectin-43 and cartilage oligomeric matrix protein were identified. In addition potential fibromodulin and biglycan peptides targeted for degradation in OA were detected. MALDI-MSI provided a novel platform to study cartilage ageing and disease enabling age and disease specific peptides in cartilage to be elucidated and spatially resolved.
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影响因子：
--
作者：
Lawrence, Reva C.;Felson, David T.;Wolfe, Frederick
通讯作者：
Wolfe, Frederick
影响因子：
4.8
作者：
Mann, HH;Özbek, S;Wagener, R
通讯作者：
Wagener, R
人体软骨中的凝集素降解--正常关节、骨关节炎关节和类风湿关节中基质金属蛋白酶和凝集素酶活性的证据
影响因子：
15.9
作者：
Lark, MW;Bayne, EK;Lohmander, LS
通讯作者：
Lohmander, LS
影响因子：
--
作者：
Cillero-Pastor, Berta;Eijkel, Gert B.;Heeren, Ron M. A.
通讯作者：
Heeren, Ron M. A.
影响因子：
5.6
作者：
Gustafsson JO;Oehler MK;Ruszkiewicz A;McColl SR;Hoffmann P
通讯作者：
Hoffmann P