Demonstration of ganglioside GD3 in human reactive astrocytes
Demonstration of ganglioside GD3 in human reactive astrocytes
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人反应性星形胶质细胞中神经节苷脂 GD3 的演示
DOI：
10.1046/j.1440-1819.1999.00475.x
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发表时间：
1999
影响因子：
11.9
通讯作者：
S. Kuroda
中科院分区：
文献类型：
--
作者：
K. Kawai;S. Watarai;H. Takahashi;H. Ishizu;K. Fukai;Y. Tanabe;O. Yokota;S. Kuroda
星形胶质细胞是积极参与中枢神经系统（CNS）损伤修复过程的细胞，在正常发育的产后中枢神经系统中发生的任何病理状况中，随着时间的推移，不同程度的反应性星形细胞增多症变得明显。使用小鼠 IgM 抗 GD3 单克隆抗体 (DSG-1)，对尸检的克雅氏病 (CJD) 和陈旧性脑梗死患者的反应性星形胶质细胞中的神经节苷脂 GD3 (II 3 a(NeuAca2-8NeuAc)-LacCer，GD3) 进行免疫细胞化学研究。 CJD 和脑梗塞中的反应性星形胶质细胞在细胞质内表现出 GD3 免疫反应性。正常星形胶质细胞呈阴性。目前的数据提出了反应性星形胶质细胞中的 GD3 对细胞特性（例如细胞运动性）具有生物学意义的可能性。
Astrocytes are the cells that actively participate in the process of lesion repair in the central nervous system (CNS), and reactive astrocytosis of varying degrees becomes apparent with time in any pathological condition occurring in the normally developed postnatal CNS. Ganglioside GD3 (II 3 a(NeuAca2‐8NeuAc)‐LacCer, GD3) in reactive astrocytes from autopsied patients with Creutzfeldt–Jakob disease (CJD) and old cerebral infarction was investigated immunocytochemically, using mouse IgM anti‐GD3 monoclonal antibody (DSG‐1). Reactive astrocytes in CJD and cerebral infarction demonstrated GD3‐immunoreactivity within the cytoplasm. Normal astrocytes were negative. The present data raise the possibility that GD3 in reactive astrocytes has biological implications for the properties of the cells, such as cellular motility.