Mapping of antigenic changes in the haemagglutinin of Hong Kong influenza (H3N2) strains using a large panel of monoclonal antibodies.
Mapping of antigenic changes in the haemagglutinin of Hong Kong influenza (H3N2) strains using a large panel of monoclonal antibodies.
复制标题
使用大量单克隆抗体绘制香港流感 (H3N2) 毒株血凝素的抗原变化图谱。
DOI：
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发表时间：
1982
影响因子：
3.8
通讯作者：
P. Underwood
中科院分区：
文献类型：
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作者：
P. Underwood
一组由125个单克隆抗体（IgG）组成，用以对抗香港（H3N2）亚型流感早期代表的血凝素。根据它们与来自同一亚型的其他16种病毒株的交叉反应，将它们分为不同的组。这种分类是用数字分类法进行的。统计检验支持分组的有效性。确定了10个这样的群体。9种抗体仍未分类。估计了影响每个抗体组结合的氨基酸残基在血凝素分子上的位置。这是通过对抗体交叉反应谱的研究，加上先前公布的不同血凝素一级序列氨基酸变化的位置，以及它们在分子三级结构中的位置来实现的。影响抗体结合的氨基酸的位置在不同的抗体组之间重叠，形成一个围绕可能的细胞受体袋的连续环。影响每个抗体基团结合的氨基酸可能代表也可能不代表实际的抗体结合位点。讨论了病毒进化过程中血凝素氨基酸变异的不同位点的重要性。
A panel of 125 monoclonal antibodies (IgG) was raised against the haemagglutinin of an early representative of the Hong Kong (H3N2) subtype of influenza. They were classified into groups based on their cross-reactions with 16 other virus strains from the same subtype. This classification was performed using methods of numerical taxonomy. Statistical tests supported the validity of the grouping. Ten such groups were identified. Nine antibodies remained unclassified. The locations on the haemagglutinin molecule of amino acid residues influencing the binding of each antibody group were estimated. This was achieved by a study of antibody cross-reactive profiles, coupled with previously published locations of amino acid changes in the primary sequence of different haemagglutinins, and their positions in the tertiary structure of the molecule. The locations of the amino acids affecting antibody binding overlapped between the different antibody groups forming a continuous ring surrounding the probable cell-receptor pocket. The amino acids affecting the binding of each antibody group may or may not represent the actual antibody binding sites. The importance of the different sites of amino acid variation in the haemagglutinin during evolution of the virus is discussed.