CLONING AND SEQUENCING OF THE SIMIAN PARVOVIRUS GENOME
CLONING AND SEQUENCING OF THE SIMIAN PARVOVIRUS GENOME
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DOI：
10.1006/viro.1995.1348
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发表时间：
1995-07-10
期刊：
影响因子：
3.7
通讯作者：
YOUNG, NS
中科院分区：
文献类型：
--
作者：
BROWN, KE;GREEN, SW;YOUNG, NS
我们最近报道了在患有严重贫血的食蟹猴中鉴定出一种新型猿细小病毒。我们现在描述病毒 DNA 的 4986 个核苷酸的克隆和测序。与人类细小病毒 B19 一样，猿细小病毒也包裹着正负单链 DNA。正链包含两个大的开放阅读框，左侧开放阅读框编码非结构蛋白，右侧阅读框编码两个衣壳蛋白。猿猴细小病毒与自主细小病毒或依赖病毒 AAV-2 几乎没有同源性，但与细小病毒 B19 DNA 总体同源性为 50%。在氨基酸水平上，与 B19 衣壳蛋白有 70% 同源性，与 B19 非结构蛋白有 50% 同源性。基于这种遗传相似性以及已知的病毒对食蟹猴红系前体的趋向性，我们建议这种新病毒应归类为细小病毒科红病毒属的新成员。
We recently reported the identification of a novel simian parvovirus in cynomolgus monkeys with severe anemia. We now describe the cloning and sequencing of 4986 nucleotides of the viral DNA. Like the human parvovirus B19, simian parvovirus encapsidates both positive and negative single-stranded DNA The positive strand contains two large open reading frames, with the left open reading frame encoding the nonstructural protein(s) and the right reading frame encoding the two capsid proteins. Simian parvovirus has little homology with the autonomous parvoviruses or the dependovirus AAV-2 but 50% overall homology with parvovirus B19 DNA. At the amino acid level there was 70% homology with B19 capsid proteins and 50% homology with B19 nonstructural protein. Based on this genetic similarity and with the known tropism of the virus for cynomolgus erythroid precursors, we suggest that this new virus should be classified as a new member of the Erythrovirus genus of the Parvoviridae.