Expanding the Burkholderia pseudomallei Complex with the Addition of Two Novel Species: Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov.
Expanding the Burkholderia pseudomallei Complex with the Addition of Two Novel Species: Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov.
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DOI：
10.1128/aem.01583-21
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发表时间：
2022-01-01
影响因子：
4.4
通讯作者：
Wagner, David M.
中科院分区：
文献类型：
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作者：
Hall, Carina M.;Baker, Anthony L.;Wagner, David M.
从热带北方澳大利亚（北领地和托雷斯海峡群岛，昆士兰州）采集的土壤样品中分离出不同的伯克霍尔德菌菌株。16 S rRNA和全基因组序列的系统发育分析显示，这些菌株是不同于以前描述的伯克霍尔德菌属物种，并将它们分配到两个新的分支内的B。类鼻疽复合体（Bpc）。由于平均核苷酸的身份和数字DNA-DNA杂交计算是一致的，这些分支代表不同的物种，我们建议的名称伯克霍尔德氏菌属种新的和伯克霍尔德氏菌属种新的菌株分配到B。mayonissp. nov.包括模式菌株BDU 6（T）（=TSD-80; LMG 29941; ASM 152374 v2）和BDU 8。菌株归属于B。savanopharynsp. nov.包括模式菌株MSMB 266（T）（=TSD-82; LMG 29940; ASM 152444 v2）、MSMB 852、BDU 18和BDU 19。比较基因组学揭示了这两个假定物种的独特编码区，包括与噬菌体相关的正向同源基因簇。型菌株均为B。mayonissp. nov.和B.在Bpc中，savanopharynsp. nov.产生的生化谱彼此不同，也不同于其他物种，并且谱在B内的菌株之间也不同。mayonissp. nov.和B.基质辅助激光解吸电离飞行时间（MLST）分析显示一个B。nov.聚簇分离，而B. mayonissp. nov.菌株没有形成明显的簇。也不是B。nov. nor B.当通过皮下途径给药时，savanopyrussp. nov.引起小鼠死亡。添加B。mayonissp. nov.和B. nov.导致目前在BPC内总共有8个物种。重要性伯克霍尔德氏菌属物种可以是新的天然产物的重要来源，并且新的物种是不同科学学科感兴趣的。虽然许多伯克霍尔德氏菌属物种是嗜血的，但类鼻疽伯克霍尔德氏菌是类鼻疽病的病原体。了解与B最接近的亲属的基因组学和毒力。假鼻疽，即，B中的其他物种。假鼻疽复合体（Bpc）对于鉴定针对B的强有力的诊断靶点非常重要。为了解B.假鼻疽两个拟新种，B. mayonissp. nov.和B. savanalypsp. nov.，从澳大利亚北方多个地点采集的土壤样品中分离得到。这两个物种属于Bpc，但在遗传学上与该复合体的所有其他成员不同。添加B。mayonissp. nov.和B. savanobactersp. nov.在这种重要的细菌复合体中总共产生了8个物种，可用于未来的研究。
Distinct Burkholderia strains were isolated from soil samples collected in tropical northern Australia (Northern Territory and the Torres Strait Islands, Queensland). Phylogenetic analysis of 16S rRNA and whole genome sequences revealed these strains were distinct from previously described Burkholderia species and assigned them to two novel clades within the B. pseudomallei complex (Bpc). Because average nucleotide identity and digital DNA-DNA hybridization calculations are consistent with these clades representing distinct species, we propose the names Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov. Strains assigned to B. mayonis sp. nov. include type strain BDU6(T) (=TSD-80; LMG 29941; ASM152374v2) and BDU8. Strains assigned to B. savannae sp. nov. include type strain MSMB266(T) (=TSD-82; LMG 29940; ASM152444v2), MSMB852, BDU18, and BDU19. Comparative genomics revealed unique coding regions for both putative species, including clusters of orthologous genes associated with phage. Type strains of both B. mayonis sp. nov. and B. savannae sp. nov. yielded biochemical profiles distinct from each other and from other species in the Bpc, and profiles also varied among strains within B. mayonis sp. nov. and B. savannae sp. nov. Matrix-assisted laser desorption ionization time-of-flight (MLST) analysis revealed a B. savannae sp. nov. cluster separate from other species, whereas B. mayonis sp. nov. strains did not form a distinct cluster. Neither B. mayonis sp. nov. nor B. savannae sp. nov. caused mortality in mice when delivered via the subcutaneous route. The addition of B. mayonis sp. nov. and B. savannae sp. nov. results in a total of eight species currently within the Bpc.IMPORTANCE Burkholderia species can be important sources of novel natural products, and new species are of interest to diverse scientific disciplines. Although many Burkholderia species are saprophytic, Burkholderia pseudomallei is the causative agent of the disease melioidosis. Understanding the genomics and virulence of the closest relatives to B. pseudomallei, i.e., the other species within the B. pseudomallei complex (Bpc), is important for identifying robust diagnostic targets specific to B. pseudomallei and for understanding the evolution of virulence in B. pseudomallei. Two proposed novel species, B. mayonis sp. nov. and B. savannae sp. nov., were isolated from soil samples collected from multiple locations in northern Australia. The two proposed species belong to the Bpc but are phylogenetically distinct from all other members of this complex. The addition of B. mayonis sp. nov. and B. savannae sp. nov. results in a total of eight species within this significant complex of bacteria that are available for future studies.