A mutation in ELA1, an age-dependent negative regulator of PAP1/MYB75, causes UV- and cold stress-tolerance in Arabidopsis thaliana seedlings
A mutation in ELA1, an age-dependent negative regulator of PAP1/MYB75, causes UV- and cold stress-tolerance in Arabidopsis thaliana seedlings
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DOI：
10.1016/j.plantsci.2009.02.010
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发表时间：
2009-05-01
期刊：
影响因子：
5.2
通讯作者：
Lee, Hojoung
中科院分区：
文献类型：
--
作者：
Choi, Seungwon;Kwon, Ye Rim;Lee, Hojoung
紫外线是陆生植物遇到的几种严重的非生物胁迫之一。它通过破坏DNA、蛋白质和脂质，损害细胞代谢并导致细胞死亡。包括各种类黄酮在内的酚类物质对紫外线照射起着“防晒霜”的作用：特别是类黄酮在吸收自由基、猝灭单线态氧和分解过氧化物方面起着关键作用。在这里。我们从拟南芥中筛选了一个类黄酮水平升高的耐紫外线突变体，并鉴定了ela1隐性突变体。我们证明，ela1突变体增强的紫外线耐受性似乎是由高水平的紫外线吸收色素（如花青素）赋予的。ela1突变体表现出作为PAP1和冷胁迫耐受性负调控因子的新特征，并且其作用特异性地存在于拟南芥发育的早期阶段。我们的研究结果清楚地表明，黄酮类化合物的生物合成受一个发育程序的调控，其中ELA1作为一个重要的调控成分参与。2009爱思唯尔爱尔兰有限公司版权所有。
UV light is one of several severe abiotic stresses encountered by terrestrial plants. It is impairing cellular metabolism and causing cell death by damaging DNA, proteins, and lipids. Phenolics including various flavonoids serve as "sunscreens" to UV exposure: in particular, flavonoids play pivotal roles in absorbing free radicals, quenching singlet oxygen, and decomposing peroxides. Here. we screened Arabidopsis thaliana for a UV-tolerant mutant with elevated flavonoid levels, and identified the recessive ela1 mutant. We demonstrate that the enhanced UV stress-tolerance shown in the ela1 mutant seems to be conferred by high levels of UV-absorbing pigments such as anthocyanins. The ela1 mutant exhibited novel characteristics as a negative regulator of PAP1 and cold stress-tolerance, and its role is specific to early stages of A. thaliana development. Our results clearly suggest that flavonoid biosynthesis is regulated by a developmental program where ELA1 participates as an important regulatory component. (C) 2009 Elsevier Ireland Ltd. All rights reserved.